PMID: 60262Aug 1, 1976Paper

Influence of steroid-receptor complexes on transcription by human hypertrophied prostatic RNA polymerases

Molecular and Cellular Endocrinology
P Davies, K Griffiths

Abstract

The effects of 5alpha-dihydrotestosterone-receptor complexes on transcription in human hypertrophied prostate tissue were studied in a cell-free system reconstituted from the various subcellular fractions prepared from specimens of the diseased gland. Two major RNA polymerase species were isolated from human hypertrophied prostate. These were designated A and B and were distinguishable by their preference for divalent cations and their sensitivity to salt and alpha-amanitin. Moreover, RNA polymerase B, but not RNA polymerase A, could effectively transcribe a prostate chromatin template. Any enzyme activity endogenous to some chromatin preparations was shown to be characteristic of RNA polymerase B. 5alpha-Dihydrotestoterone-receptor complexes were transferred into prostatic chromatin both steroid- and tissue-specifically. The association of steroid-receptor complexes with chromatin produced changes in template activity and increased the transcription of the chromatin by exogenous and endogenous RNA polymerase B. With a number of specimens, however, there was considerable variation in accessible cytoplasmic receptor sites, uptake of steroid-receptor complexes by chromatin preparations, the template activity of the chromatin and ...Continue Reading

References

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Feb 9, 1963·JAMA : the Journal of the American Medical Association·W E FARNSWORTH, J R BROWN

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Citations

Jan 1, 1995·International Urology and Nephrology·V L Kumar, P K Majumder
Feb 17, 2001·The Journal of Steroid Biochemistry and Molecular Biology·R TuftsZ Wang
Feb 1, 1986·British Journal of Urology·N KyprianouK Griffiths

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