Influence of strain on [3H]thymidine incorporation, surfactant-related phospholipid synthesis, and cAMP levels in fetal type II alveolar cells

American Journal of Respiratory Cell and Molecular Biology
J E ScottJ E Anderson

Abstract

Episodic fetal breathing movements occur in utero; however, their purpose is not known. While some evidence indicates these movements produce distention of the fetal lung, the effects of eliminating the movements on lung development are not clear. Using preparations of the surfactant-producing fetal rabbit lung type II cells, we have examined the hypothesis that distention is involved in maturation of these cells as determined by replication rate and phospholipid synthesis. Differentiating type II cells were isolated from fetal rabbit lungs on gestational day 24. After allowing 24 h for the cells to attach, distention (10%) was applied using a Flexercell apparatus at a rate of 3 s of strain and 57 s of rest for 24 or 48 h. Cells were concurrently incubated with [3H]thymidine. Autoradiography showed that [3H]thymidine-labeled nuclei of fetal type II cells and fibroblasts could be identified. Strain significantly increased incorporation of the radioisotope into DNA. In the remaining studies, cells were grown to confluence prior to use. Cell viability after straining (3 or 50 cycles per minute [cpm] for 48 h) was assessed by incubating with diacetyl fluorescein/ethidium bromide and viewing by fluorescence microscopy. Straining did...Continue Reading

References

May 23, 2001·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Y S Edwards
Jul 5, 2011·American Journal of Physiology. Lung Cellular and Molecular Physiology·Lynn M CrosbyC M Waters
Jul 1, 1999·American Journal of Respiratory Cell and Molecular Biology·M E De PaepeF I Luks
Nov 17, 2007·Proceedings of the National Academy of Sciences of the United States of America·Dongeun HuhShuichi Takayama
Mar 1, 1993·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J E AndersonJ E Scott
Oct 21, 2003·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Ashley C WozniakJudy E Anderson
Feb 2, 2017·Cell and Tissue Research·Johannes C Schittny
Nov 11, 2006·The Journal of Pediatrics·Daphne J JanssenLuc J Zimmermann
Jun 1, 1996·Pediatric Pulmonology·P J MerkusP H Quanjer
Mar 25, 2008·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Stefan SchumannJosef Guttmann
Jun 6, 2006·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y WangJ Sanchez-Esteban
Jun 17, 2020·Biotechnology and Bioengineering·Milad RadiomYong Chen
Aug 13, 1999·Journal of Applied Physiology·C M WatersJacob I Sznajder
Apr 12, 2002·American Journal of Physiology. Lung Cellular and Molecular Physiology·Eduardo Correa-MeyerJacob I Sznajder
Feb 4, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Sanjeev Kumar MahtoJosué Sznitman
Apr 27, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·T NakamuraM Post
Oct 12, 1999·The American Journal of Physiology·M LiuM Post
Jul 13, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·P R ChessJ N Finkelstein
Jun 16, 1999·Journal of Applied Physiology·D J Tschumperlin, S S Margulies
Jul 18, 2001·Journal of Applied Physiology·J Sanchez-EstebanL P Rubin
May 15, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·K J HaleyC M Lilly
Oct 29, 2000·Journal of Applied Physiology·M Liu, M Post
Jun 14, 2012·International Journal of Molecular Medicine·Kozue KobayashiHidenori Inaoka
Nov 15, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·Y S EdwardsA W Murray
Nov 18, 2000·American Journal of Physiology. Cell Physiology·P G De Deyne
Jun 12, 2018·Biomicrofluidics·Joseph W SongDongeun Huh
Dec 9, 1998·The American Journal of Physiology·D J Tschumperlin, S S Margulies
Dec 9, 1998·The American Journal of Physiology·J PuginJ C Chevrolet

Citations

Dec 1, 1975·Journal of Applied Physiology·E J MescherW H Tooley
Mar 1, 1979·Early Human Development·J S Wigglesworth, R Desai
Mar 15, 1978·American Journal of Obstetrics and Gynecology·J PatrickR Voegelin
Jul 1, 1987·Journal of Applied Physiology·B J BuckleyB A Freeman
Jan 1, 1966·Proceedings of the National Academy of Sciences of the United States of America·B Rotman, B W Papermaster
Apr 1, 1984·Environmental Health Perspectives·S A Rooney
Jan 3, 1980·Biochimica Et Biophysica Acta·D SomjenA Harell
Jan 1, 1984·Calcified Tissue International·I BindermanD Somjen
Sep 20, 1983·Biochimica Et Biophysica Acta·J E ScottP G Harding
Mar 1, 1980·The American Review of Respiratory Disease·G SmithR Bone
Apr 1, 1982·American Journal of Obstetrics and Gynecology·S Neldam
Feb 15, 1980·American Journal of Obstetrics and Gynecology·J PatrickB Richardson
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

Related Concepts

Choline
Fetal Structures
Stress, Mechanical
Fetal Lung
Specimen Type - Fibroblasts
Pulmonary Alveolar Structure
Microvilli
Surfactant [EPC]
Endogenous Surfactants
Lung

Related Feeds

American Thoracic Association Journals

Discover the latest respiratory research published by the journals from the American Thoracic Society.