PMID: 9644231Jun 30, 1998Paper

Influence of substrate structure on turnover of the organic cation/H+ exchanger of the renal luminal membrane

Pflügers Archiv : European journal of physiology
S H Wright, T M Wunz

Abstract

We examined the influence of organic cation (OC) structure on the rate of turnover of the OC/H+ exchanger in rabbit renal brush-border membrane vesicles (BBMV). The rate of efflux of [14C]tetraethylammonium ([14C]TEA) from BBMV, measured in the presence of an inwardly directed chemical gradient for test agent, provided an indirect measure of activity of the OC/H+(OC) exchanger. The trans-stimulation of [14C]TEA efflux from BBMV was a saturable function of increasing extravesicular concentration of both unlabeled TEA and tetramethylammonium (TMA), with an apparent Michaelis constant (Kt) for the interaction of these compounds with the OC/H+(OC) exchanger of 25 microM and 1 mM, respectively. The effect on [14C]TEA efflux of saturating extravesicular concentrations of a series of n-tetraalkylammonium compounds was examined. Whereas the short-chain compounds TMA and TEA markedly stimulated [14C]TEA efflux (by 830% and 690%, respectively), the long-chain compounds tetrapropylammonium and tetrabutylammonium were less effective, increasing efflux by only 40% and 120%, respectively. When the exchanger was saturated with tetrapentylammonium, mediated efflux of [14C]TEA was reduced. Increasing alkyl chain length was also correlated with ...Continue Reading

Citations

Mar 16, 2012·The Journal of Pharmacology and Experimental Therapeutics·Bethzaida AstorgaStephen H Wright
Feb 7, 2002·The Journal of Pharmacy and Pharmacology·Takatoshi TakuboHiroyasu Ogata
Apr 20, 2007·American Journal of Physiology. Renal Physiology·Xiaohong ZhangStephen H Wright
Feb 29, 2008·American Journal of Physiology. Cell Physiology·Takuya MatsumotoYoshinori Moriyama

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