Influence of surface charge on the rate, extent, and structure of adsorbed Bovine Serum Albumin to gold electrodes

Journal of Colloid and Interface Science
Burcu BeykalMeagan S Mauter

Abstract

The objective of this work is to investigate the rate, extent, and structure of amphoteric proteins with charged solid surfaces over a range of applied potentials and surface charges. We use Electrochemical Quartz Crystal Microbalance with Dissipation Monitoring (E-QCM-D) to investigate the adsorption of amphoteric Bovine Serum Albumin (BSA) to a gold electrode while systematically varying the surface charge on the adsorbate and adsorbent by manipulating pH and applied potential, respectively. We also perform cyclic voltammetry-E-QCM-D on an adsorbed layer of BSA to elucidate conformational changes in response to varied applied potentials. We confirm previous results demonstrating that increasing magnitude of applied potential on the gold electrode is positively correlated with increasing mass adsorption when the protein and the surface are oppositely charged. On the other hand, we find that the rate of BSA adsorption is not governed by simple electrostatics, but instead depends on solution pH, an observation not well documented in the literature. Cyclic voltammetry with simultaneous E-QCM-D measurements suggest that BSA protein undergoes a conformational change as the surface potential varies.

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Citations

Jun 15, 2019·International Journal of Environmental Research and Public Health·Yuman Zhou, Xiaorong Gao
Oct 21, 2016·Physiological Research·M KumorekT Riedel
May 24, 2017·Materials Science & Engineering. C, Materials for Biological Applications·Ghader HosseinzadehAli A Moosavi-Movahedi
Jul 24, 2021·ACS Applied Materials & Interfaces·Ruth F Balderas-Valadez, Claudia Pacholski
Jul 26, 2017·Environmental Science & Technology·Soumya PanditMoshe Herzberg

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