Abstract
Currently two factors hinder the use of collagen as building block of regenerative devices: the limited mechanical strength in aqueous environment, and potential antigenicity. Polymeric collagen is naturally found in the cross-linked state and is mechanically tougher than the monomeric, acid-soluble collagen ex vivo. The antigenicity of collagen, on the other hand, is mainly ascribed to inter-species variations in amino acid sequences of the non-helical terminal telopeptides. These telopeptides can be removed through enzymatic treatment to produce atelocollagen, although the effect of this cleavage on triple helix organization, amino acidic composition and thermal properties is often disregarded. Here, we compare the structural, chemical and physical properties of polymeric and monomeric type I collagen with and without telopeptides, in an effort to elucidate the influence of either mature covalent crosslinks or telopeptides. Circular dichroism (CD) was used to examine the triple helical conformation and quantify the denaturation temperature (Td) of both monomeric collagen (36.5°C) and monomeric atelocollagen (35.5°C). CD measurements were combined with differential scanning calorimetry (DSC) in order to gain insight into the t...Continue Reading
Citations
Aug 21, 2018·Advanced Materials·Anna SorushanovaDimitrios I Zeugolis
Jan 19, 2020·Polymers·Chie KojimaAkikazu Matsumoto
May 3, 2019·Communications Biology·Priti Prasanna MaitySantanu Dhara
Sep 1, 2020·Expert Review of Molecular Diagnostics·Steffen K MeurerRalf Weiskirchen
Mar 1, 2019·Marine Drugs·Wen-Kui SongHu Hou
Dec 20, 2018·PloS One·Johanna RoetherNorbert Willenbacher
Feb 27, 2020·Antioxidants·Gabriel Aguirre-CruzGabriel Aguirre-Álvarez
May 12, 2020·Tissue Engineering. Part C, Methods·Robert A PouliotRebecca L Heise
Aug 6, 2020·Materials·Alexey VeremeevAnton Kutikhin
Nov 10, 2020·International Journal of Biological Macromolecules·Yaqin RanXudong Li
Jan 20, 2021·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Murat KazanciGihan Kamel
Nov 25, 2020·Pharmaceutics·Alessandra GirottiFrancisco Javier Arias
Feb 28, 2021·Journal of Biomedical Materials Research. Part a·A Hernández-Rangel, E San Martin-Martinez
Jan 12, 2020·Communications Biology·Priti Prasanna MaitySantanu Dhara
Oct 15, 2020·Marine Drugs·Ching-Shu LaiMei-Ling Tsai
May 18, 2021·International Biomechanics·Marco Fielder, Arun K Nair
May 25, 2021·Food Chemistry·Xiaoyue YuYang Hu
Jul 3, 2021·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Anna PudłoWiesław Kopeć
Jul 25, 2021·Polymers·Ruth NaomiFezah Othman
Jul 9, 2021·Journal of Biomedical Materials Research. Part a·Leigh SlykerLawrence J Bonassar
Nov 17, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Ken TeraoMasahiro Abe
Aug 27, 2021·Membranes·Jie YinGiuseppe Tronci
Aug 28, 2021·International Journal of Molecular Sciences·Hanna LewandowskaMarcin Moniuszko