Influence of temperature on the detectibility and chromosomal distribution of specific DNA sequences by in situ hybridisation

Chromosoma
M H MoarK W Jones

Abstract

Hybridising certain AT-rich satellite complementary RNAs (cRNAs) to their homologous chromosomal DNA sequences at different temperatures of incubation results in a different dispersion of autoradiographic label throughout the karyotypes. The temperature at which most label, or cRNA-DNA hybrid formation, exists corresponds to the optimal rate temperature for the hybridisation of these same satellite cRNA-DNA hybrids as determined by RNA excess filter hybridisation. It is likely that the in situ hybridisation results can therefore be explained by the fact that there is a similar temperature-dependence on the rate of hybrid formation for both in situ and RNA excess hybridisation. This should have important implications for the designing of in situ hybridisation experiments in general.

References

Jan 1, 1971·Cytogenetics·F E Arrighi, T C Hsu
Oct 27, 1971·Nature: New Biology·K W Jones, G Corneo
Jan 1, 1973·International Review of Cytology·W Hennig
Jan 1, 1974·Cytogenetics and Cell Genetics·D M Kurnit
Jan 1, 1974·Chromosoma·K W JonesG Corneo
Jan 14, 1972·Journal of Molecular Biology·M L BirnstielI F Purdom
Jun 19, 1973·Biochemical and Biophysical Research Communications·J R Hutton, J G Wetmur
Jul 18, 1973·Chromosoma·K W JonesE Ginelli
Jun 15, 1973·Journal of Molecular Biology·C A ThomasB M Dancis
Nov 1, 1973·The Biochemical Journal·B D Young, J Paul
May 5, 1974·Journal of Molecular Biology·A Shenkin, R H Burdon
Jan 1, 1969·Progress in Nucleic Acid Research and Molecular Biology·P M Walker
Jun 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·J G Gall, M L Pardue
Jul 1, 1965·Journal of Molecular Biology·D Gillespie, S Spiegelman
Aug 1, 1969·Biochemistry·B L McConaughyB J McCarthy
Nov 19, 1971·Biochimica Et Biophysica Acta·G CorneoE Polli
Jun 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·W G FlammP M Walker
Jul 21, 1971·Nature: New Biology·W D Sutton, M McCallum
Mar 14, 1970·Journal of Molecular Biology·G CorneoE Polli
Jun 12, 1970·Science·M L Pardue, J G Gall
Apr 14, 1968·Journal of Molecular Biology·G CorneoE Polli
Feb 1, 1968·Gan no rinsho. Japan journal of cancer clinics·Y Miura, N Fukui
Aug 9, 1969·Nature·H A JohnK W Jones
Jan 15, 1963·Proceedings of the National Academy of Sciences of the United States of America·J VINOGRADW F DOVE

❮ Previous
Next ❯

Citations

Mar 22, 1978·Chromosoma·L Manuelidis
Jan 1, 1980·Human Genetics·E M Bühler
Dec 6, 1976·Chromosoma·L SinghK W Jones
Sep 1, 1981·The Histochemical Journal·J G Bauman, P van Duijn
Jun 22, 1978·Biochimica Et Biophysica Acta·M H Moar, G Klein
Nov 27, 1981·Biochimica Et Biophysica Acta·J ProsserP C Vincent
Dec 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·M Brahic, A T Haase
Nov 1, 1976·Nucleic Acids Research·L Manuelidis
Mar 1, 1979·Experimental Cell Research·E BarberáC Alonso
Nov 25, 1979·Journal of Molecular Biology·A R MitchellC J Bostock
May 25, 2006·International Journal of Hematology·Kazuo Kinoshita, Taichiro Nonaka

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)
G Terenghi, J M Polak
Current Topics in Pathology. Ergebnisse Der Pathologie
G Terenghi, R A Fallon
The Veterinary Record
W G Hill
© 2022 Meta ULC. All rights reserved