Influence of testosterone on polyunsaturated fatty acid biosynthesis in Sertoli cells in culture

Cell Biochemistry and Function
Graciela E Hurtado de Catalfo, I N T de Gómez Dumm

Abstract

Sertoli cells play a central role in spermatogenesis, its development and regulation. They are target cells for androgen action in the seminiferous tubules. The Sertoli cell is considered to be the most important cell type in the testis with regard to essential fatty acid metabolism. We studied the response to testosterone of cultured Sertoli cells from immature rats by determining the fatty acid composition of total cellular lipids as well as by the biosynthesis of polyunsaturated fatty acids. Fatty acid methyl esters were analysed by gas liquid chromatography and radiochromatography. Two doses of testosterone were tested (150 and 300 ng ml(-1)). Significant differences were found in fatty acids derived from total cellular lipids after 8 days in culture in the presence of testosterone (300 ng ml(-1), for 48 h). Compared to controls, the hormone produced a significant increase of 16:1 and 18:1 n-9, and of 18:2 n-6, and a decrease of 20:4 and 22:5 n-6 in total cellular lipids. The decrease in the n-6 fatty acid ratios 20:4/20:3, 20:4/18:2 and 24:5/24:4, and the increase in 18:1n-9/18:0 and 16:1n-9/16:0 ratios were taken as an indirect signal of testosterone effects on Delta5, Delta6 and Delta9 desaturase activities. The drop in ...Continue Reading

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Citations

May 3, 2012·Nature Reviews. Urology·Luís RatoPedro F Oliveira
Feb 20, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Thomas SaetherTrine B Haugen
Sep 11, 2014·Molecular Human Reproduction·Cátia S RochaMarco G Alves
Sep 27, 2012·Cellular and Molecular Life Sciences : CMLS·Marco G AlvesPedro F Oliveira
Aug 5, 2011·International Journal of Andrology·P F OliveiraS Socorro
Nov 22, 2016·Journal of Animal Physiology and Animal Nutrition·K StarčevićT Mašek
Mar 20, 2012·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Ramazan BalEbru Etem

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