PMID: 9182720Jun 1, 1997Paper

Influence of the conserved disulphide bond, exposed to the putative binding pocket, on the structure and function of the immunoglobulin-like molecular chaperone Caf1M of Yersinia pestis

The Biochemical Journal
V P Zav'yalovV M Abramov

Abstract

The Yersinia pestis protein Caf1M is a typical representative of a subfamily of periplasmic molecular chaperones with characteristic structural and functional features, one of which is the location of two conserved cysteine residues close to the putative binding pocket. We show that these residues form a disulphide bond, the reduction and alkylation of which significantly increases the dissociation constant of the Caf1M-Caf1 (where Caf 1 is a polypeptide subunit of the capsule) complex [from a Kd of (4.77+/-0.50)x10(-9) M for the intact protein to one of (3.68+/-0.68)x10(-8) M for the modified protein]. The importance of the disulphide bond for the formation of functional Caf1M in vivo was demonstrated using an Escherichia coli dsbA mutant carrying the Y. pestis f1 operon. In accordance with the CD and fluorescence measurements, the disulphide bond is not important for maintenance of the overall structure of the Caf1M molecule, but would appear to affect the fine structural properties of the subunit binding site. A three-dimensional model of the Caf1M-Caf1 complex was designed based on the published crystal structure of PapD (a chaperone required for Pap pili assembly) complexed with a peptide corresponding to the C-terminus of...Continue Reading

Citations

Jul 28, 2009·Journal of Thrombosis and Haemostasis : JTH·P J Hogg
Mar 23, 2007·Molecular Microbiology·Anton V Zavialov, Stefan D Knight
Jun 20, 2007·FEMS Microbiology Reviews·Anton ZavialovVladimir Zav'yalov
Sep 27, 2005·Microbes and Infection·Dongsheng ZhouRuifu Yang
Aug 16, 2002·Molecular Microbiology·A V ZavialovS D Knight
Sep 14, 2010·Antioxidants & Redox Signaling·Iman AzimiPhilip J Hogg
Feb 10, 2009·Nature Reviews. Microbiology·Begoña HerasJennifer L Martin
Jul 26, 2017·The Journal of Biological Chemistry·Justyna PilipczukRafał Piątek
Apr 3, 2001·Applied and Environmental Microbiology·A V ZavialovV P Zav'yalov
Apr 1, 2005·The Biochemical Journal·Anton V ZavialovStefan D Knight
Mar 15, 2018·EcoSal Plus·Glenn T Werneburg, David G Thanassi

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