PMID: 8944332Jan 1, 1996Paper

Influence of the extraction procedure on plasminogen activator inhibitor-2 (PAI-2) and urokinase receptor (uPAR) assays in breast cancer tissues

Breast Cancer Research and Treatment
C Bouchet-BernetJ Oglobine

Abstract

The levels of uPA, its inhibitors PAI-1 and PAI-2, and the uPA receptor (uPAR) have prognostic value in breast cancer. However, different extraction methods and assays kits are used in different laboratories and may directly influence the levels observed. To define a buffer suitable for both PAI-2 and uPAR extraction from breast cancer tissue compatible with hormone receptors and other cytosolic prognosticator assays, we compared PAI-2 and uPAR values obtained by immunoenzymatic assays (American Diagnostica, Greenwhich, USA) in several extraction conditions: 1) cytosol obtained with the standard hormone receptor buffer; 2) solubilized pellets obtained by Triton X100 extraction of the pelleted membranes obtained with standard hormone receptor buffer; 3) cytosol obtained by direct extraction in the buffer (containing Triton X100) recommended by the manufacturer, after 2 hours or 12 hours of incubation. Cytosol extracts prepared using the standard procedure recommended for hormone receptors gave the highest PAI-2 values. The highest uPAR values were obtained in the subsequent detergent extraction of the pelleted membranes. PAI-2 levels obtained with the kit manufacturer's method after 12 hours of incubation were lower than those o...Continue Reading

References

Aug 19, 1992·Journal of the National Cancer Institute·F SpyratosM Brunet
Feb 1, 1992·International Journal of Cancer. Journal International Du Cancer·K SumiyoshiS Baba
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Citations

Mar 5, 1999·Breast Cancer Research and Treatment·R W StephensM Schmitt
Feb 22, 2011·Human Reproduction·Luis A RamónAmparo Estellés
Apr 5, 2003·Biological Chemistry·Ami S BhattCharles S Craik
Mar 13, 2014·Human Reproduction·Aitana Braza-BoïlsJuan Gilabert-Estellés
Sep 25, 2010·Annals of the New York Academy of Sciences·Bruce S McEwen
Oct 3, 1999·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·C BouchetF Spyratos

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