Influence of the L-arginine-nitric oxide pathway on vasoactive intestinal polypeptide release and motility in the rat stomach in vitro

European Journal of Pharmacology
S WillisV Schumpelick

Abstract

Endogenous nitric oxide (NO) plays an important role as non-adrenergic, non-cholinergic inhibitory transmitter in the gastrointestinal tract, especially in sphincter regions. The aim of this study was to investigate the influence of NO on pyloric motility and on the release of vasoactive intestinal polypeptide (VIP) in the isolated perfused rat stomach in vitro. Therefore, pyloric motility was continuously recorded by a special sleeve manometry catheter placed in the pyloric region and the concentration of VIP was determined in the venous effluent of the portal vein. Arterial perfusion with the nitrate agonist sodiumnitroprusside led to a dose-dependent reduction of the pyloric motility index (basal 166 +/- 48 mm Hg/min: sodiumnitroprusside 10(-6) M 30 +/- 20 mmHg/min: sodiumnitroprusside 10(-4) M 0: n = 8. P < 0.001) while VIP release was not influenced significantly. Inhibition of endogenous NO production by the NO-synthase inhibitor NG-nitro-L-Arg (L-NNA) significantly increased pyloric motility (basal motility index 175 +/- 28 mmHg/min: L-NNA 10(-4) M 348 +/- 48 mmHg/min: n = 8, P < 0.05). This effect was completely blocked by addition of L-Arg 10(-3) M (125 +/- 45 mm Hg/min: n = 8, P < 0.01), L-NNA and L-Arg both did not i...Continue Reading

References

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Citations

Dec 25, 2002·Clinical Biochemistry·Samar K KassimAnwar Abd El Mohsen
Jun 6, 2003·European Journal of Pharmacology·Erin SymondsTaher Omari
Mar 19, 1999·European Journal of Pharmacology·S J CzuczwarZ Kleinrok
Jul 26, 2002·Regulatory Peptides·Florian LipplHans Dieter Allescher
Nov 11, 1998·General Pharmacology·D Currò, P Preziosi
Jul 23, 2002·Autonomic Neuroscience : Basic & Clinical·Lille-Mor Lindeström, Eva Ekblad

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