Influence of the local helical conformation on the guanine modifications generated from one-electron DNA oxidation

Biochemistry
A Spassky, D A Angelov

Abstract

Two major products, 2,2-diamino-4-[(2-deoxy-beta-D-erythro-pentafuranosyl)amino]-5-(2H )-oxazolone and its imidazole derivative have been generated from one-electron oxidation of the free 2'-deoxyguanosine. The formation of 7,8-dihydro-8-oxoguanine (8-oxodG), not detected in this case, has been observed from DNA exposed to oxidizing agents. Since these compounds are thought to reflect, respectively, either deprotonation or hydration of the transient guanyl radical cation, these findings suggested that the helical structure could influence the chemical decomposition pathway of the guanine moiety. In the present study, we have photoionized DNA sequences by exposure to high-intensity UV (266 nm) laser pulses. Homo- or heteroduplexes, including guanines in various environments as well as Gn runs, were used as templates. Lesions were analyzed, at the nucleotide level, by taking advantage of the specific removal of 8-oxodG from DNA by the formamidopyrimidine DNA glycosylase (Fpg protein) and of the differential sensitivity of 8-oxodG and oxazolone to piperidine. Variations were observed in the relative yield of each type of lesion at individual guanines of the DNA sequences. We found that the Fpg lesions predominate in regions of sta...Continue Reading

References

May 20, 1992·Journal of Molecular Biology·L Joshua-TorJ L Sussman
Aug 1, 1989·Quarterly Reviews of Biophysics·O Kennard, W N Hunter
Jan 1, 1994·Annual Review of Biochemistry·B Demple, L Harrison

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Citations

Mar 25, 2011·Journal of Molecular Histology·Sandra Regina MirandaDaniel Araki Ribeiro
Feb 8, 2003·Archives of Biochemistry and Biophysics·Kazutaka HirakawaShosuke Kawanishi
Oct 13, 2012·Journal of the American Chemical Society·Kok Seong LimPeter C Dedon
Nov 23, 2011·Chemical Research in Toxicology·Kok Seong LimPeter C Dedon
Jan 16, 2007·Nature Structural & Molecular Biology·Simon AmiardMarie-Josèphe Giraud-Panis
Apr 25, 2012·Physical Chemistry Chemical Physics : PCCP·Yang LiuVladimir Shafirovich
Jul 22, 1998·Nucleic Acids Research·S KoizumeE Ohtsuka
Oct 25, 2003·Nucleic Acids Research·J R MilliganJ F Ward
Mar 15, 2011·Nucleic Acids Research·Santiago Cuesta-LópezMichel Peyrard
Aug 25, 2000·Molecular and Cellular Biology·C LemercierS Khochbin
Aug 25, 2012·Journal of Toxicology·Damian G DeavallRuth Roberts
Jun 6, 2006·Nature Chemical Biology·Yelena MargolinPeter C Dedon
Aug 12, 2008·Free Radical Biology & Medicine·Miral DizdarogluPawel Jaruga
Jun 10, 2011·Chembiochem : a European Journal of Chemical Biology·Young-Ae LeeVladimir Shafirovich
Jan 27, 2012·Free Radical Research·Miral Dizdaroglu, Pawel Jaruga
Dec 29, 2013·International Journal of Radiation Biology·Jean CadetNicholas E Geacintov
May 1, 1999·Archives of Biochemistry and Biophysics·Y HirakuS Kawanishi
Jan 11, 2011·Mutation Research·Thomas B KrystonAlexandros G Georgakilas
Mar 2, 2013·The International Journal of Neuropsychopharmacology·Márcio Gerhardt Soeiro-de-SouzaRicardo Alberto Moreno
May 21, 2016·Frontiers in Immunology·Aleli Salazar-RamiroBenjamín Pineda
Nov 5, 2014·PloS One·Isabel Lopes CorreiaIsabel de Sá-Nogueira
Nov 1, 2018·Photochemistry and Photobiology·Jean CadetDimitar Angelov
Jan 21, 2009·Journal of Physics. Condensed Matter : an Institute of Physics Journal·M PeyrardD Angelov
Apr 25, 2007·Free Radical Research·Kimiko ItoShosuke Kawanishi
Jun 8, 2006·Neurochemical Research·Hatasu KobayashiShosuke Kawanishi
Apr 30, 2016·Neurotoxicity Research·Muhammad Ummear RazaMeng-Yang Zhu
Jul 23, 2004·The Journal of Biological Chemistry·Dimitar AngelovStefan Dimitrov
Feb 8, 2019·Oxidative Medicine and Cellular Longevity·Caroline C FariaRodrigo S Fortunato
Jun 6, 2003·Journal of the American Chemical Society·Chu-Sheng Liu, Gary B Schuster

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