Influence of the M3-M4 intracellular domain upon nicotinic acetylcholine receptor assembly, targeting and function.

British Journal of Pharmacology
S KracunNeil S Millar

Abstract

The aim of this study was to investigate the influence of the intracellular domain of nicotinic acetylcholine receptor (nAChR) subunits upon receptor assembly, targeting and functional properties. Because most nAChR subunits form functional receptors only as heteromeric complexes, it can be difficult to examine the influence of individual subunits or subunit domains in isolation. A series of subunit chimaeras was constructed which contain the intracellular loop region (located between the M3 and M4 transmembrane domains) from nAChR subunits alpha1-alpha10 or beta1-beta4. All of these chimaeras contain common extracellular and transmembrane domains (from the nAChR alpha7 subunit and the 5-hydroxytryptamine receptor 5-HT(3A) subunit, respectively), thereby facilitating both homomeric receptor assembly and detection with radiolabelled or fluorescent alpha-bungarotoxin. The nAChR M3-M4 intracellular loop domain had no significant effect upon levels of total subunit protein detected in transfected cells but had a significant influence upon levels of both cell surface and intracellular assembled receptors. Comparisons of functional properties revealed a significant influence of the intracellular loop domain upon both single-channel c...Continue Reading

References

Jun 1, 1995·Trends in Neurosciences·W N Green, N S Millar
Jan 1, 1993·Journal of Cell Science. Supplement·M J de Hoop, C G Dotti
Jun 10, 1993·Nature
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·C N ConnollyS J Moss
Jan 1, 1995·Cold Spring Harbor Symposia on Quantitative Biology·I Mellman
May 1, 1997·Journal of Neurochemistry·S J LansdellN S Millar
May 29, 1998·Journal of Neurochemistry·S T Cooper, N S Millar
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·A B ElgoyhenJ Boulter
Nov 24, 2001·Trends in Cell Biology·W J Nelson, C Yeaman
May 17, 2003·The Journal of Biological Chemistry·Gary W BoydChristopher N Connolly
Jul 31, 2003·Biochemical Society Transactions·N S Millar
May 29, 2004·Trends in Neurosciences·Henry A LesterDennis A Dougherty
Apr 25, 2006·Biochimica Et Biophysica Acta·Moniek van BeestPeter M T Deen
Oct 4, 2006·Philosophical Transactions. Series A, Mathematical, Physical, and Engineering Sciences·Helen Roberts, T J Millar
Feb 7, 2007·British Journal of Pharmacology·S P H AlexanderJ A Peters

❮ Previous
Next ❯

Citations

Feb 4, 2014·Biochemical Pharmacology·Roger L Papke
Sep 14, 2013·The Journal of Biological Chemistry·Jane E CarlandJeremy J Lambert
Dec 29, 2010·The Journal of General Physiology·Rahul SrinivasanHenry A Lester
Sep 29, 2011·The Journal of General Physiology·Nicole K McKinnonMyles H Akabas
Jan 8, 2009·Physiological Reviews·Edson X AlbuquerqueScott W Rogers
Jul 23, 2014·ACS Chemical Biology·Kristina N-M DaefflerDennis A Dougherty
Nov 5, 2013·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Nadine KabbaniDavid J Adams
Jun 26, 2009·Journal of Molecular Evolution·JacSue KehoeJonathan Bradley
Nov 26, 2010·Pflügers Archiv : European journal of physiology·Claudia MoriconiFrancesca Grassi
Jul 25, 2014·The Journal of Biological Chemistry·Ying WangHenry A Lester
Aug 15, 2019·The Journal of General Physiology·Akash PandhareMichaela Jansen
Sep 2, 2014·Parasite : Journal De La Société Française De Parasitologie·José Miguel Flores-FernándezMoisés Martínez-Velázquez
Sep 18, 2018·Pakistan Journal of Biological Sciences : PJBS·Wuyi Liu, Kunkai Su
Feb 12, 2013·Molecular Neurobiology·Jianliang ZhangXiaomin Wang
Feb 23, 2021·The Journal of Biological Chemistry·Josip IvicaLucia G Sivilotti

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.