Influence of the microporous substratum and hydrodynamics on resistances to drug transport in cell culture systems: calculation of intrinsic transport parameters

Journal of Pharmaceutical Sciences
H Yu, P J Sinko

Abstract

Although cell culture models are increasingly used to study drug transport and metabolism, the influence of the substratum on the transport properties of the cell monolayer has not been studied in great detail. Furthermore, the use of effective (or apparent) permeabilities (Peff) assumes that the contribution of the microporous filter substratum and the aqueous boundary layer (ABL) to transport are negligible or are at least constant for a series of drugs. In the present study, the permeabilities of the substratum, ABL, and monolayer were obtained for a series of compounds at variable flow rates in side-by-side diffusion chambers. Comparisons of transport properties were made between cell monolayers grown on substrata made of polycarbonate (PC) and polyester (PE). All paracellular markers demonstrated a reduction in permeability and a corresponding increase in transepithelial electrical resistance (TEER) through PE-grown monolayers. The permeabilities of two carrier-mediated compounds, phenylalanine and proline, were 55% higher and 48% lower through PE-grown monolayers than through the PC-grown monolayers, respectively. The resistance to progesterone transport attributed to the PE and PC filters was large (71% and 27% of total ...Continue Reading

References

Mar 2, 1992·Biochimica Et Biophysica Acta·P L NicklinM Mackay
Apr 1, 1990·European Journal of Drug Metabolism and Pharmacokinetics·G Wilson
Sep 21, 1990·Biochimica Et Biophysica Acta·I J Hidalgo, R T Borchardt
Sep 1, 1990·Pharmaceutical Research·A R HilgersP S Burton
Jan 17, 1989·Biochimica Et Biophysica Acta·P G Plagemann, C Woffendin
Nov 14, 1986·Biochemical and Biophysical Research Communications·E S Jakobs, A R Paterson
May 1, 1983·Quarterly Reviews of Biophysics·T J Pedley
Mar 1, 1995·Medicinal Research Reviews·K M HillgrenR T Borchardt
Dec 1, 1993·The American Journal of Physiology·O CandiaT Yorio

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Citations

Apr 17, 2008·Pharmaceutical Research·Timo KorjamoJukka Mönkkönen
Aug 9, 2003·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Isabel Behrens, Thomas Kissel
Dec 23, 2009·Molecular Pharmaceutics·Xinyuan ZhangGus R Rosania
Oct 14, 2006·The Journal of Pharmacology and Experimental Therapeutics·Anand BalakrishnanJames E Polli
Sep 26, 2012·Scandinavian Journal of Gastroenterology·Eleonora BjörkmanLars Fändriks
Aug 5, 2009·Journal of Pharmaceutical Sciences·Timo KorjamoJukka Mönkkönen
Jan 23, 2013·Journal of Pharmacological and Toxicological Methods·André Bersani DezaniCristina Helena Dos Reis Serra
Mar 24, 2010·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Aki T HeikkinenTimo Korjamo
Sep 23, 2014·European Journal of Pharmaceutical Sciences : Official Journal of the European Federation for Pharmaceutical Sciences·Siti R YusofN Joan Abbott
May 1, 2016·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Thaisa Marinho DezaniCristina Helena Dos Reis Serra
Feb 18, 2005·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Cindy Q XiaLiang-Shang Gan
Feb 24, 2001·Annals of the New York Academy of Sciences·E GuptaE Rubin
Mar 7, 2009·Chemical Reviews·Stefan Balaz
Jun 2, 2007·Journal of Pharmaceutical Sciences·Donna A Volpe

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