PMID: 9166421Jun 2, 1997Paper

Influence of the NH2-terminal amino acid of the T cell receptor alpha chain on major histocompatibility complex (MHC) class II + peptide recognition

The Journal of Experimental Medicine
J L SeibelJ Kappler

Abstract

The alpha/beta T cell receptor (TCR) recognizes peptide fragments bound in the groove of major histocompatibility complex (MHC) molecules. We modified the TCR alpha chain from a mouse T cell hybridoma and tested its ability to reconstitute TCR expression and function in an alpha chain-deficient variant of the hybridoma. The modified alpha chain differed from wild type only in its leader peptide and mature NH2-terminal amino acid. Reconstituted cell surface TCR complexes reacted normally with anti-TCR and anti-CD3 antibodies. Although cross-linking of this TCR with an antibody to the TCR idiotype elicited vigorous T cell hybridoma activation, stimulation with its natural MHC + peptide ligand did not. We demonstrated that this phenotype could be reproduced simply by substituting the glutamic acid (E) at the mature NH2 terminus of the wild type TCR alpha chain with aspartic acid (D). The substitution also dramatically reduced the affinity of soluble alpha/beta-TCR heterodimers for soluble MHC + peptide molecules in a cell-free system, suggesting that it did not exert its effect simply by disrupting TCR interactions with accessory molecules on the hybridoma. These results demonstrate for the first time that amino acids which are no...Continue Reading

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Citations

Apr 11, 2003·Molecular Immunology·Wesley P ThayerPeter E Jensen
Sep 11, 2007·Nature Immunology·James P Scott-BrowneLaurent Gapin
Sep 22, 1998·The Journal of Experimental Medicine·J Q RussellR C Budd
Jul 24, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ritu Sarin, Clara Abraham
May 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·C P LiuJ Kappler
Apr 6, 2007·Biomolecular Engineering·Sarah A Richman, David M Kranz
Jan 25, 2005·Journal of Molecular Biology·Lukasz K ChlewickiDavid M Kranz
Aug 6, 2019·International Journal of Cancer. Journal International Du Cancer·David BassanLea Eisenbach
Aug 5, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·E BoenJ Bill
Mar 23, 2004·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jun LuEsteban Celis
Mar 14, 2002·International Reviews of Immunology·S CasaresT D Brumeanu

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