Influence of the partitioning of osmolytes by the cytoplasm on the passive response of cells to osmotic loading.

Biophysical Journal
Michael B AlbroGerard A Ateshian

Abstract

Due to the dense organization of organelles, cytoskeletal elements, and protein complexes that make up the intracellular environment, it is likely that membrane-permeant solutes may be excluded from a fraction of the interstitial space of the cytoplasm via steric restrictions, electrostatic interactions, and other long-range intermolecular forces. This study investigates the hypothesis that the intracellular partitioning of membrane-permeant solutes manifests itself as a partial volume recovery in response to hyperosmotic loading, based on prior theoretical and biomimetic experimental studies. Osmotic loading experiments are performed on immature bovine chondrocytes using culture conditions where regulatory volume responses are shown to be insignificant. Osmotic loading with membrane-permeant glycerol (92 Da) and urea (60 Da) are observed to produce partial volume recoveries consistent with the proposed hypothesis, whereas loading with 1,2-propanediol (76 Da) produces complete volume recovery. Combining these experimental results with the previous theoretical framework produces a measure for the intracellular partition coefficient of each of these solutes. At 1000 mOsm, 1,2-propanediol is the only osmolyte to yield a partition ...Continue Reading

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Citations

Aug 31, 2011·Biomechanics and Modeling in Mechanobiology·Siru M TurunenRami K Korhonen
Mar 10, 2010·Annals of Biomedical Engineering·Gerard A AteshianClark T Hung
Sep 29, 2011·Journal of Biomechanical Engineering·Gerard A AteshianJeffrey A Weiss
Aug 21, 2012·Mechanics Research Communications·Gerard A AteshianClark T Hung
Aug 12, 2018·Journal of Biomechanical Engineering·Jay C HouGerard A Ateshian
Jun 19, 2013·Journal of Biomechanical Engineering·Gerard A AteshianJeffrey A Weiss
Sep 16, 2021·The EMBO Journal·Florian GeigerAlexander Borodavka

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