PMID: 15228121Jul 2, 2004Paper

Influence of the pH on separating DNA by high-speed microchip electrophoresis

Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry
Tomo OsafuneYoshinobu Baba

Abstract

Various factors are critical in resolving DNA molecules at high speed, including the separation medium, concentration, composition, and pH of the buffer, as well as the electric field strength. To this study, considered the composition of a buffer and the difference in the pH, while paying attention to whether the separation ability changes in the microchip electrophoresis of DNA. DNA separation was particularly affected by both the buffer composition and the pH. Under the optimal microchip electrophoresis conditions that were determined in this study, an improved resolution of a wider range of DNA fragment sizes was achieved. Moreover, the total separation time decreased from 240 s to 100 s. Thus, by simplifying and improving the DNA electrophoresis in the microchip, this technique is now widely applicable to several different scientific fields.

References

Jan 28, 1999·Analytical Chemistry·I Kheterpal, R A Mathies
Feb 24, 2001·Electrophoresis·M N Albarghouthi, A E Barron
Feb 24, 2001·Analytical Chemistry·E T LagallyR A Mathies
Dec 26, 2001·Biophysical Journal·Noritada KajiYoshinobu Baba
Sep 5, 2002·Electrophoresis·Masanori UedaYoshinobu Baba

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Citations

Feb 11, 2012·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Zacchary D KihmYan Liu

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