Influence of the respirator on volatile organic compounds: an animal study in rats over 24 hours

Journal of Breath Research
Frederic W AlbrechtSascha Kreuer

Abstract

Long-term animal studies are needed to accomplish measurements of volatile organic compounds (VOCs) for medical diagnostics. In order to analyze the time course of VOCs, it is necessary to ventilate these animals. Therefore, a total of 10 male Sprague-Dawley rats were anaesthetized and ventilated with synthetic air via tracheotomy for 24 h. An ion mobility spectrometry coupled to multi-capillary columns (MCC-IMS) was used to analyze the expired air. To identify background contaminations produced by the respirator itself, six comparative measurements were conducted with ventilators only. Overall, a number of 37 peaks could be detected within the positive mode. According to the ratio peak intensity rat/ peak intensity ventilator blank, 22 peaks with a ratio >1.5 were defined as expired VOCs, 12 peaks with a ratio between 0.5 and 1.5 as unaffected VOCs, and three peaks with a ratio <0.5 as resorbed VOCs. The peak intensity of 12 expired VOCs changed significantly during the 24 h measurement. These results represent the basis for future intervention studies. Notably, online VOC analysis with MCC-IMS is possible over 24 h in ventilated rats and allows different experimental approaches.

References

Aug 1, 1995·Free Radical Research·S MendisD E Euler
Mar 25, 1995·Lancet·H E Lebovitz
Feb 1, 1996·Journal of Applied Physiology·W M FosterT H Risby
Aug 18, 2004·Clinica Chimica Acta; International Journal of Clinical Chemistry·Wolfram MiekischGabriele F E Noeldge-Schomburg
May 15, 2007·The Science of the Total Environment·Hung-Lung ChiangYao-Sheng Huang
Oct 16, 2007·Journal of Hazardous Materials·H M ZhuK F Cen
Mar 31, 2009·Analytical and Bioanalytical Chemistry·Sasidhar MaddulaJörg Ingo Baumbach
Jan 16, 2010·Journal of Applied Physiology·Wolfgang VautzMarcus Peters
Sep 28, 2010·Journal of Theoretical Biology·Julian KingAnton Amann
Jun 1, 2008·Journal of Breath Research·Wolfram MiekischJochen K Schubert
Jul 23, 2011·Journal of Applied Physiology·Stephanie NeuhausMarcus Peters
Jan 3, 2012·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Ana V GuamánRamon Farré
Jul 14, 2012·Analytica Chimica Acta·Marco RighettoniSotiris E Pratsinis
Sep 20, 2012·Journal of Breath Research·Nhât-Nam Le-DongAnh Tuan Dinh-Xuan
Apr 1, 2014·Journal of Breath Research·Tobias FinkSascha Kreuer
May 20, 2014·Journal of Breath Research·F MaurerS Kreuer
Oct 18, 2014·The European Respiratory Journal·Heather D BeanJane E Hill

❮ Previous
Next ❯

Citations

Oct 27, 2018·Critical Care Medicine·Tobias HüppeSascha Kreuer
Feb 7, 2019·Medizinische Klinik, Intensivmedizin und Notfallmedizin·L M WirtzT Hüppe
Apr 28, 2017·The European Respiratory Journal·Ildiko HorváthTeunis J Vink
Sep 30, 2016·Journal of Breath Research·Astrid KleberTobias Fink
Mar 3, 2020·Anesthesia and Analgesia·Lukas M Müller-WirtzSascha Kreuer
Apr 13, 2021·Advanced Biology·Aylen Lisset Jaimes-MogollónRadu Ionescu
Aug 17, 2021·Journal of Analytical Methods in Chemistry·Tobias HüppeSascha Kreuer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.