Influence of thermally driven surface undulations on tethers formed from bilayer membranes.

Biophysical Journal
Emily Glassinger, Robert M Raphael

Abstract

Tether formation is a powerful method to study the mechanical properties of soft lipid bilayer membranes. The force required to maintain a tether at a given length depends upon both membrane elastic properties and tension. In this report, we develop a theoretical analysis that considers the contribution of thermally driven surface undulations and the corresponding entropically driven tensions on the conformation of tethers formed from unaspirated lipid vesicles. In this model, thermal undulations of the vesicle surface provide the excess area required for tether formation. Energy minimization demonstrates the dependence of equilibrium tether conformation on membrane tension and provides an analytical relationship between tether force and radius. If the contributions of nonlocal bending are not considered, an analytical relationship between tether force and length can also be obtained. The predictions of the model are compared to recently reported experimental data, and a value for the initial vesicle tension is obtained. Since most analyses of tether formation from cells and unaspirated vesicles neglect the contributions of nonlocal bending, the appropriateness of this assumption is analyzed. The effect of surface microvesicula...Continue Reading

References

Jan 1, 1990·Advances in Biophysics·H Hotani, H Miyamoto
Sep 1, 1987·Biophysical Journal·R E Waugh, R M Hochmuth
Jan 1, 1972·Transactions - American Society for Artificial Internal Organs·R M HochmuthD W Johnson
May 1, 1995·Annals of Biomedical Engineering·R E Waugh, R G Bauserman
Jan 1, 1996·Biophysical Journal·F M HochmuthM P Sheetz
Nov 1, 1987·Physical Review A: General Physics·S T Milner, S A Safran
Jun 1, 1994·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics·L MiaoH G Döbereiner
Nov 1, 1996·Physical Review. E, Statistical Physics, Plasmas, Fluids, and Related Interdisciplinary Topics· Jan Bukman DM Wortis
Apr 23, 1990·Physical Review Letters·E Evans, W Rawicz
Mar 1, 1993·Physical Review Letters·U Seifert
May 15, 1999·Science·B M DischerD A Hammer
Jun 27, 2000·Biophysical Journal·W RawiczE Evans
May 2, 2001·Nature Reviews. Molecular Cell Biology·M P Sheetz
Jun 1, 2001·Physical Review Letters·J B FournierL Peliti
Dec 12, 2001·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·R M RaphaelB Zeks
Apr 18, 2002·Proceedings of the National Academy of Sciences of the United States of America·Aurélien RouxPatricia Bassereau
May 15, 2002·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Thomas R PowersRaymond E Goldstein
May 23, 2002·Biophysical Journal·Robert M Hochmuth, Warren D Marcus
Jun 13, 2002·Physical Review Letters·Imre DerényiJacques Prost
Mar 27, 2003·Proceedings of the National Academy of Sciences of the United States of America·Arpita UpadhyayaAlexander van Oudenaarden
Dec 10, 2003·Proceedings of the National Academy of Sciences of the United States of America·Gerbrand KosterMarileen Dogterom
Apr 28, 2004·Biophysical Journal·Arpita Upadhyaya, Michael P Sheetz
Jun 1, 2004·Physical Review Letters·Ana-Suncana SmithUdo Seifert
Jun 14, 2005·Biophysical Journal·Yong Zhou, Robert M Raphael

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Citations

Jul 10, 2012·Biophysical Journal·Svetlana BaoukinaD Peter Tieleman
May 11, 2010·Physical Biology·Sarah A Nowak, Tom Chou
Nov 13, 2009·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Kristopher R SchumacherAlexander A Spector

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