Influence of thyroidal status on pituitary content of thyrotropin beta- and alpha-subunit, growth hormone, and prolactin messenger ribonucleic acids

Molecular Endocrinology
C J MirellJ M Hershman

Abstract

Thyroidectomized rats were used to study the effects of a single injection of T3 on pituitary mRNA synthesis and hormone secretion. T3 was injected ip at doses of 0, 0.2, 1, or 5 micrograms/100 g body weight, and and animals were killed 24 h later. T3 caused a significant decrease in serum TSH, but caused no significant change in either serum GH or PRL. Pituitary mRNA was quantified by slot blot hybridization with cDNA probes specific for alpha-TSH, beta-TSH, PRL, and GH. We found that both the alpha and beta mRNA subunits decreased, that PRL mRNA remained relatively unchanged, and that GH mRNA increased with increasing T3 dose. The data show that a single dose of T3 can profoundly influence mRNA levels in the anterior pituitary; the lowest dose of T3 caused maximum inhibition of alpha-TSH mRNA while beta-TSH mRNA declined further in a dose-dependent manner.

Citations

Aug 3, 1999·The Journal of Clinical Investigation·E D AbelF Wondisford
Mar 17, 2007·Critical Reviews in Toxicology·R Thomas ZoellerRochelle W Tyl
Mar 15, 2003·Risk Analysis : an Official Publication of the Society for Risk Analysis·R Thomas Zoeller
Nov 23, 2006·Annals of the New York Academy of Sciences·R Thomas Zoeller

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