PMID: 6885251Jul 1, 1983Paper

Influence of trifluoroacetic acid on retention times of histidine-containing tryptic peptides in reverse phase HPLC

International Journal of Peptide and Protein Research
A S AcharyaJ M Manning


The tryptic peptides of the aminoethylated alpha- and beta-chain of hemoglobin have been separated on a Partisil-10 ODS-2 column with a linear gradient of acetonitrile containing 0.1% trifluoroacetic acid. The elution profile of the tryptic peptides of the chains obtained with this acetonitrile trifluoroacetate system has been compared with that obtained using phosphoric acid as the ion-pairing reagent. This comparison demonstrated that trifluoroacetate influences the retention times of the histidine-containing tryptic peptides much more than it affects those peptides that do not contain histidine residues. This behavior has been rationalized on the basis of ion-pairing of trifluoroacetate with the histidine residues of the tryptic peptides.


Dec 1, 1989·Journal of Protein Chemistry·C VerdeA Parente
Sep 1, 1989·International Journal of Peptide and Protein Research·R SeyerB Castro
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·C Cirotto, A Parente
Jul 16, 1985·Biochemical and Biophysical Research Communications·P PucciL Pagano
Dec 30, 1986·Biochemistry·A Di DonatoG D'Alessio
Aug 27, 1985·Biochemistry·A S Acharya, R Seetharam

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