PMID: 3753881Feb 27, 1986Paper

Influence of vesicle size on complement-dependent immune damage to liposomes

Biochimica Et Biophysica Acta
R L RichardsC R Alving

Abstract

Complement-dependent antibody-mediated damage to multilamellar lipid vesicles (MLVs) normally results in a maximum release of 50-60% of trapped aqueous marker. The most widely accepted explanation for this is that only the outermost lamellae of MLVs are attacked by complement. To test this hypothesis, complement damage to two different types of large unilamellar vesicles (LUVs), large unilamellar vesicles prepared by the reverse-phase evaporation procedure (REVs) and large unilamellar vesicles prepared by extrusion techniques (LUVETs), were determined. In the presence of excess antibody and complement the LUVs released a maximum of only approx. 25 to 40% of trapped aqueous marker, instead of close to 100% that would be expected. Since small unilamellar vesicles apparently differ from LUVs in that they can release 100% of trapped aqueous marker it appeared that the size of the vesicles was an important factor. Because of these observations the influence of MLV size on marker release was examined. Three populations of MLVs of different sizes were separated by a fluorescence activated cell sorter. Assays of the separated MLV populations showed that the degree of complement-dependent marker release was inversely related to MLV size...Continue Reading

References

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Citations

Sep 29, 1989·Journal of Immunological Methods·T MasakiH Okada
Jun 6, 2000·Advanced Drug Delivery Reviews·A J Bradley, D V Devine
Mar 16, 2001·International Journal of Pharmaceutics·T M HuongH Kiwada
Oct 6, 2004·Journal of Immunoassay & Immunochemistry·O D HendricksonB B Dzantiev
Dec 20, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tyson SmythThomas J Anchordoquy
Jan 30, 2013·Drug Development and Industrial Pharmacy·Guanyu ChenJingyuan Wen
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Jan 10, 1989·Biochemistry·J A Malinski, G L Nelsestuen
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Mar 1, 1988·Chemical & Pharmaceutical Bulletin·U GlagasigijY Suzuki
Mar 28, 2021·Journal of Controlled Release : Official Journal of the Controlled Release Society·Julian VoglerShyh-Dar Li
Apr 20, 1994·Biochimica Et Biophysica Acta·D V DevineP R Cullis

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