Influence of whole body irradiation and local shielding on matrix-induced endochondral bone differentiation

Calcified Tissue International
S WientroubA H Reddi

Abstract

Subcutaneous implantation of demineralized bone matrix into allogeneic rats induces endochondral bone formation. We have investigated the effects of irradiation on the sequelae of the interaction of collagenous matrix and mesenchymal cells and on cartilage and bone differentiation. Rats were irradiated in a vertical direction with a midline dose of 850 rad. Radiation entered the rats ventrally while a small area of the upper thorax was locally shielded. After irradiation, bone matrix was implanted in shielded and nonshielded sites, and the implants were studied at various stages. On day 3, [3H]thymidine incorporation, an index of cell proliferation, was inhibited by 70% in the nonshielded sites compared to nonirradiated control rats. The degree of inhibition (35%) was less pronounced in shielded sites. Furthermore, there was recovery of cell proliferation in the shielded sites as opposed to the nonshielded contralateral site. A similar pattern was observed on day 7 as assessed by 35SO4 incorporation into proteoglycans during chondrogenesis. Bone formation and mineralization were quantified on day 11 by alkaline phosphatase activity and 45Ca incorporation. In nonshielded sites, there was a 73% inhibition of alkaline phosphatase ...Continue Reading

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Citations

Apr 1, 1988·Calcified Tissue International·S Wientroub, A H Reddi
Feb 5, 2004·International Journal of Oral and Maxillofacial Surgery·M I AlamS Enomoto
Sep 8, 2001·Plastic and Reconstructive Surgery·M I AlamS Enomoto
Dec 1, 1993·In Vitro Cellular & Developmental Biology. Animal·L Wolfinbarger, Y Zheng
Sep 16, 2006·Journal of Materials Science. Materials in Medicine·P Q RuhéJ A Jansen
Jan 26, 1999·Clinical Orthopaedics and Related Research·A H Reddi
Mar 27, 2001·The Journal of Bone and Joint Surgery. American Volume·A H Reddi

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