Influences of starch and sucrose on Streptococcus mutans biofilms

Oral Microbiology and Immunology
S DuarteH Koo

Abstract

The combination of starch and sucrose has been shown to be potentially more cariogenic than either alone. The aim of this study was to examine the influence of starch and sucrose, alone or in combinations, on formation, polysaccharide composition, gene expression, and acidogenicity of Streptococcus mutans biofilms. S. mutans UA159 biofilms were formed on saliva-coated hydroxyapatite (sHA) discs in batch culture for 5 days in the presence of 1% (weight/volume) starch, 1% sucrose, 1% starch plus 1% sucrose, 1% starch plus 0.5% fructose plus 0.5% glucose, or 1% sucrose plus 1% glucose. Amylase activity from sHA disks was detected up to 48 h, thereby increasing the availability of reducing sugars and acidogenicity in the early stages of biofilm development. S. mutans grown in the presence of sucrose alone or in combinations formed well-defined and tightly adherent biofilms comprised of mostly water-insoluble polysaccharides (INS); in contrast, the presence of starch or starch + glucose + fructose resulted in little biofilm formation with minimal amounts of INS. However, the combination of starch + sucrose produced biofilms with more biomass and acidogenicity, and a higher content of INS than those grown in sucrose or sucrose + gluc...Continue Reading

References

Apr 1, 1992·Journal of Dental Research·D T ZeroL M Gwinner
Sep 18, 1991·Carbohydrate Research·D T Fu, J F Robyt
Jan 1, 1989·Archives of Oral Biology·I Al-Hashimi, M J Levine
Jun 1, 1987·Scandinavian Journal of Dental Research·A A ScheieG Rölla
May 1, 1980·The Journal of the American Dental Association·W H BowenM F Cole
Jan 1, 1993·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·F A ScannapiecoM J Levine
Feb 1, 1996·Journal of Dental Research·M S LamkinF G Oppenheim
Mar 10, 2000·Archives of Oral Biology·A M Vacca Smith, W H Bowen
Oct 6, 2000·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·P LingströmS Kashket
Oct 31, 2002·The Journal of the American Dental Association·William H Bowen
Mar 7, 2003·Applied and Environmental Microbiology·Thomas ThurnheerBernhard Guggenheim
Oct 8, 2003·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Howard K Kuramitsu
Aug 24, 2005·The British Journal of Nutrition·Cecilia C C RibeiroJaime A Cury
Jan 27, 2006·Antimicrobial Agents and Chemotherapy·Hyun KooRobert G Quivey
Sep 26, 2006·Journal of Dental Research·A F Paes LemeJ A Cury
May 4, 2007·Analytical Biochemistry·Jaime A Cury, Hyun Koo

❮ Previous
Next ❯

Citations

Oct 17, 2013·MBio·Brandon W PetersonPrashant K Sharma
Feb 13, 2016·Archives of Oral Biology·Clarissa Gewehr SteguesLina Naomi Hashizume
Dec 4, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Christopher S AdamsPaul Ducheyne
Mar 8, 2016·Journal of Dentistry·Sufian A YassinNicholas S Jakubovics
Jun 18, 2014·American Journal of Physical Anthropology·Mark C Griffin
Nov 6, 2009·Cellular Microbiology·Céline LoussertAnne Beauvais
Jun 7, 2015·International Journal of Biological Macromolecules·Małgorzata PleszczyńskaJanusz Szczodrak
Jul 12, 2013·Evidence-based Complementary and Alternative Medicine : ECAM·Marcos Guilherme da CunhaPedro Luiz Rosalen
May 2, 2013·Archives of Oral Biology·Rodrigo A GiacamanRamiro J Castro
May 28, 2014·FEMS Microbiology Letters·Emeline BouffartiguesSylvie Chevalier
Apr 16, 2014·Biofouling·Yuri Wanderley CavalcantiJaime Aparecido Cury
Jul 13, 2016·European Journal of Dentistry·Natalia Díaz-GarridoRodrigo A Giacaman
Aug 1, 2016·Archives of Oral Biology·Remberto Marcelo Argandoña ValdezCristiane Duque
May 26, 2016·Brazilian Oral Research·Juliana Nunes BotelhoJaime Aparecido Cury
Oct 26, 2014·Applied and Environmental Microbiology·Erica Shapiro Frenkel, Katharina Ribbeck
Mar 14, 2019·Brazilian Dental Journal·Gabriela RezendeLina Naomi Hashizume
Aug 1, 2015·PloS One·Denise Lins de SousaSimone Duarte
Feb 15, 2018·Caries Research·Samilly Evangelista SouzaJaime Aparecido Cury
Mar 7, 2019·Royal Society Open Science·Luis Pezo-LanfrancoAndré Carlo Colonese
Jun 21, 2020·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Ramille Araújo LimaTereza De Jesus Pinheiro Gomes Bandeira
May 15, 2019·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Erika R H KawakitaCarolina P Aires
Aug 23, 2020·Applied and Environmental Microbiology·Natalia Díaz-GarridoRodrigo A Giacaman
Jan 27, 2021·Photodiagnosis and Photodynamic Therapy·Dhara Yanka Tiburtino GaldinoMarco Aurélio Benini Paschoal
Dec 16, 2020·Scientific Reports·Mi-Ah KimKyung-San Min
Mar 9, 2021·Dental Materials Journal·Sabrina FeitosaSimone Duarte
Mar 20, 2021·Nature Microbiology·Caroline A WerlangKatharina Ribbeck

❮ Previous
Next ❯

Related Concepts

Related Feeds

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.