Influences on the lifespan of B cell subpopulations defined by different phenotypes

European Journal of Immunology
D A Fulcher, A Basten

Abstract

The turnover of mature and immature B cells defined by a range of cell surface markers was investigated by feeding normal or bcl-2-transgenic (bcl-2-Tg) mice 5'-bromo-2-deoxyuridine (BrdUrd) for up to 6 weeks. In peripheral lymphoid tissue, B cells accumulated BrdUrd with a 50% labeling time of 4.3 weeks and a pattern of uptake indicative of the presence of both long-lived and short-lived cells. These two kinetic populations could be resolved into immature B220lo/heat-stable antigen (HSA)hi cells which labeled rapidly, and B220hiHSAlo cells which were uniformly long-lived with a half-life of about 6 weeks. During loading and pulse-chase experiments, BrdUrd uptake by cells within the mature B220hiHSAlo population clearly followed an exponential kinetic pattern, suggesting that their loss was governed by stochastic processes. Using other surface markers, the long-lived population could also be defined by high expression of IgD, representing cells in the follicular mantle zone of the spleen, and by the phenotype IgMhiIgDloHSAlo which most likely represented marginal zone memory B cells. CD23 expression on B cells did not differentiate well between long and short-lived cells. Only about half of newly labeled B cells appearing in th...Continue Reading

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