Influenza a virus induces an immediate cytotoxic activity in all major subsets of peripheral blood mononuclear cells.

PloS One
Sanda SturlanM Bergmann

Abstract

A replication defective influenza A vaccine virus (delNS1 virus) was developed. Its attenuation is due to potent stimulation of the innate immune system by the virus. Since the innate immune system can also target cancer cells, we reasoned that delNS1 virus induced immune-stimulation should also lead to the induction of innate cytotoxic effects towards cancer cells. Peripheral blood mononuclear cells (PBMCs), isolated CD56+, CD3+, CD14+ and CD19+ subsets and different combinations of the above subsets were stimulated by delNS1, wild type (wt) virus or heat inactivated virus and co-cultured with tumor cell lines in the presence or absence of antibodies against the interferon system. Stimulation of PBMCs by the delNS1 virus effectively induced cytotoxicity against different cancer cell lines. Surprisingly, virus induced cytotoxicity was exerted by all major subtypes of PBMCs including CD56+, CD3+, CD14+ and CD19+ cells. Virus induced cytotoxicity in CD3+, CD14+ and CD19+ cells was dependent on virus replication, whereas virus induced cytotoxicity in CD56+ cells was only dependent on the binding of the virus. Virus induced cytotoxicity of isolated cell cultures of CD14+, CD19+ or CD56+ cells could be partially blocked by antibodie...Continue Reading

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Citations

Sep 3, 2011·Journal of Clinical Bioinformatics·Soumyaroop BhattacharyaThomas J Mariani
Feb 6, 2015·BMC Complementary and Alternative Medicine·Jing LiuChunying Li

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Methods Mentioned

BETA
flow cytometry
FCS
density gradient centrifugation
Assay
ELISA

Software Mentioned

EXPO32

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