Influenza A/H3N2 virus infection in immunocompromised ferrets and emergence of antiviral resistance

PloS One
Rueshandra RoosenhoffRon A M Fouchier

Abstract

Influenza viruses can cause severe life threatening infections in high-risk patients, including young children, the elderly and patients with compromised immunity due to underlying medical conditions or immunosuppressive treatment. The impaired immunity of these patients causes prolonged virus infection and combined with antiviral treatment facilitates the emergence of viruses with resistance mutations. The diverse nature of their immune status makes them a challenging group to study the impact of influenza virus infection and the efficacy of antiviral therapy. Immunocompromised ferrets may represent a suitable animal model to assess influenza virus infection and antiviral treatment strategies in immunocompromised hosts. Here, ferrets were given a daily oral solution of mycophenolate mofetil, tacrolimus and prednisolone sodium phosphate to suppress their immune system. Groups of immunocompromised and immunocompetent ferrets were inoculated with an A/H3N2 influenza virus and were subsequently treated with Oseltamivir or left untreated. Quantitative real-time reverse transcription polymerase chain reaction (qRT-PCR) was performed on the throat and nose specimens to study virus replication during the course of infection. All immun...Continue Reading

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Citations

Sep 29, 2019·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Rueshandra RoosenhoffPieter L A Fraaij
Jun 14, 2020·Influenza and Other Respiratory Viruses·Mitsutaka KitanoAkira Naito
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Nov 8, 2020·Antiviral Research·Hassan ZaraketNelson Lee
Jun 18, 2021·Infectious Diseases·Ville KailaJaana Syrjänen

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Datasets Mentioned

BETA
CY114485
CY114487

Methods Mentioned

BETA
PCR
Assay

Software Mentioned

CLC genomic Workbench

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