Influenza reverse genetics: dissecting immunity and pathogenesis

Expert Reviews in Molecular Medicine
Siying YeJohn Stambas

Abstract

Reverse genetics systems allow artificial generation of non-segmented and segmented negative-sense RNA viruses, like influenza viruses, entirely from cloned cDNA. Since the introduction of reverse genetics systems over a decade ago, the ability to generate 'designer' influenza viruses in the laboratory has advanced both basic and applied research, providing a powerful tool to investigate and characterise host-pathogen interactions and advance the development of novel therapeutic strategies. The list of applications for reverse genetics has expanded vastly in recent years. In this review, we discuss the development and implications of this technique, including the recent controversy surrounding the generation of a transmissible H5N1 influenza virus. We will focus on research involving the identification of viral protein function, development of live-attenuated influenza virus vaccines, host-pathogen interactions, immunity and the generation of recombinant influenza virus vaccine vectors for the prevention and treatment of infectious diseases and cancer.

References

Jan 1, 1990·Annual Review of Immunology·I A Wilson, N J Cox
Jun 12, 1989·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·P M ColmanR G Webster
Dec 1, 1989·Journal of Virology·J D ParvinM Krystal
Aug 11, 1993·Nucleic Acids Research·A ZobelG Hobom
Oct 15, 1996·Proceedings of the National Academy of Sciences of the United States of America·P PaleseA García-Sastre
Dec 24, 1996·Proceedings of the National Academy of Sciences of the United States of America·A Bridgen, R M Elliott
May 23, 1998·Annual Review of Immunology·M M DavisY Chien
Feb 17, 1999·Proceedings of the National Academy of Sciences of the United States of America·A H ReidJ K Taubenberger
Jun 8, 1999·Annual Review of Immunology·J W Yewdell, J R Bennink
Aug 4, 1999·Proceedings of the National Academy of Sciences of the United States of America·G NeumannY Kawaoka
Sep 30, 1999·Drugs & Aging·E M Corrigan, R L Clancy
May 10, 2000·Proceedings of the National Academy of Sciences of the United States of America·E HoffmannR G Webster
Jun 6, 2000·The Journal of Experimental Medicine·G E PriceD Moskophidis
Aug 31, 2000·Annual Review of Biochemistry·J J Skehel, D C Wiley
Sep 20, 2000·The Journal of General Virology·S GoodbournR E Randall
Dec 1, 2000·Zeitschrift Für Naturforschung. C, a Journal of Biosciences·M MilevaS Ribarov
Feb 15, 2001·The Journal of Infectious Diseases·L V GubarevaF G Hayden

❮ Previous
Next ❯

Citations

Jul 30, 2015·Virus Research·Aitor NogalesLuis Martínez-Sobrido
Jan 31, 2015·The Journal of General Virology·Tian-Cheng LiReimar Johne
Mar 31, 2015·BioMed Research International·Ernest Milián, Amine A Kamen
Apr 15, 2020·PLoS Pathogens·Luis Martinez-SobridoStacey Schultz-Cherry

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antivirals (ASM)

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antivirals

Antivirals are medications that are used specifically for treating viral infections. Discover the latest research on antivirals here.

Related Papers

Virology
G Neumann, Yoshihiro Kawaoka
Methods in Molecular Biology
Chang-Won Lee, David L Suarez
© 2021 Meta ULC. All rights reserved