Influenza virus budding does not require a functional AAA+ ATPase, VPS4.

Virus Research
Rie Watanabe, Robert A Lamb

Abstract

The process of budding of many enveloped viruses utilizes the cellular ESCRT (endosomal sorting complex required for transport) machinery, that is normally involved in the formation of luminal vesicles of endosomal multivesiculate bodies (MVB). A late step in the MVB pathway involves the recruitment of VPS4, an AAA+ ATPase, to the ESCRT complexes. Our earlier work had shown that the formation of influenza virus-like particles was not inhibited by dominant negative VPS4A. However, it was not known if there was a role of VPS4 and the ESCRT pathway in influenza virus particle budding and this needed to be investigated. It was found that neither siRNA knockdown of VPS4A and VPS4B expression nor the use of cell lines that inducibly express VPS4A or VPS4B dominant negative mutants, inhibited influenza virus budding. In contrast, and in keeping with more recent data, vesicular stomatitis virus budding was diminished by VPS4 dysfunction.

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Citations

Jun 15, 2011·Viruses·Mark Yondola, Carol Carter
Oct 9, 2013·Annual Review of Cell and Developmental Biology·Jeremy S Rossman, Robert A Lamb
Nov 12, 2014·Proceedings of the National Academy of Sciences of the United States of America·Nina F RoseJohn K Rose
Apr 16, 2013·Current Opinion in Virology·Winfried WeissenhornPatricia Bassereau
Feb 1, 2012·Cellular Microbiology·Dimiter DemirovStephan Ludwig
Jan 20, 2012·The Journal of General Virology·Emily A BrucePaul Digard
Apr 16, 2015·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Baoying HuChunhua Wan
Nov 28, 2012·Biochemical Society Transactions·Emily A BrucePaul Digard
Jan 18, 2011·Virology·Jeremy S Rossman, Robert A Lamb
Dec 24, 2014·Experimental and Molecular Pathology·Dongmei ZhangQiyun Tang
Mar 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Takeo GoraiYoshihiro Kawaoka
Aug 28, 2020·Frontiers in Cellular and Infection Microbiology·Yuan JiangChuanshan Xu
Jun 21, 2017·Biophysical Journal·Charles E JacksonPhyllis I Hanson

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