Feb 1, 1988

Influenza virus ISCOMs: biochemical characterization

Vaccine
B SundquistB Morein

Abstract

Immunostimulating complexes (ISCOMs) have been prepared from influenza A virus envelope glycoproteins, i.e. haemagglutinin (HA) and neuraminidase (NA). An ISCOM consists of a matrix, which is the micellar form of the glycoside, Quil A, in hydrophobic interaction with both the envelope glycoproteins (HA/NA). The Quil A bound to the ISCOM amounted to 50 micrograms mg-1 (5%) of ISCOM protein. ISCOMs were morphologically identified as symmetrical cage-like structures of approximately equal to 40 nm in diameter with hexagonal or pentagonal subunits of approximately equal to 12 nm. The sedimentation coefficient was approximately equal to 19 S as compared to 30 S for the glycoprotein micelles. The biological activities of the HA and NA are preserved in both ISCOMs and micelles.

Mentioned in this Paper

Corona Virus Membrane Protein E1
Immunologic Adjuvants
Glycoproteins
SDS-PAGE
Quil A
Influenza
Myxoviruses
Cardiac Glycosides
Centrifugation
Neuraminidase

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