Influx of extracellular calcium mediates 1,25-dihydroxyvitamin D3-dependent transcaltachia (the rapid stimulation of duodenal Ca2+ transport)

Endocrinology
A R de Boland, Anthony W Norman

Abstract

We investigated the role of extracellular Ca2+ in 1,25-dihydroxyvitamin D3 [1,25-(OH)2D3] rapid stimulation of intestinal Ca2+ transport (termed transcaltachia) in the perfused duodenal of vitamin D-replete chicks. The carboxylic ionophore ionomycin (2 microM) was found to stimulate 45Ca2+ transport from the lumen to the vascular effluent to the same extent as physiological levels of 1,25-(OH)2D3. The increase in duodenal 45Ca2+ transport caused by 1,25-(OH)2D3 was dependent on the presence of medium Ca2+, since it was abolished by prior addition of EGTA and was restored upon the addition of Ca2+. Depolarization of the basal lateral membrane of intestinal epithelial cells with 70 mM K+ caused a rapid increase in 45Ca2+ transport (30% above control values within 2 min and 250% after 20 min of vascular perfusion). The rise was also abolished by prior addition of EGTA. Intracellular calcium concentrations ([Ca2+]i) were measured in isolated duodenal cells from vitamin D-replete chicks using the fluorescent dye fura 2. A 1-min incubation with physiological concentrations of 1,25-(OH)2D3 (130 pM) caused an increase in [Ca2+]i from a basal level of 168 +/- 23 nM to 363 +/- 44 nM. Pretreatment of intestinal epithelial cells with the p...Continue Reading

Citations

Feb 1, 2000·Journal of Cellular Physiology·V MassheimerA R De Boland
Jun 23, 2005·Diabetologia·C MathieuR Bouillon
Mar 15, 1995·Thrombosis Research·M E MillerG M Dores
Sep 1, 1991·Molecular and Cellular Endocrinology·I Nemere, A W Norman
Mar 1, 1992·The Journal of Steroid Biochemistry and Molecular Biology·A W NormanM C Farach-Carson
Feb 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·D W BrannV B Mahesh
Oct 31, 1991·Biochemical and Biophysical Research Communications·R L BolandE Ritz
Mar 26, 1998·Biochemical Pharmacology·R Gniadecki
Apr 18, 2002·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Francis SunnyOommen V Oommen
Aug 14, 2002·The British Journal of Dermatology·J E Osborne, P E Hutchinson
Jul 5, 2008·Annual Review of Nutrition·Ramesh C Khanal, Ilka Nemere
Jul 17, 2013·Nutrition Journal·Natielen Jacques SchuchLígia Araújo Martini
Aug 13, 2008·Endocrine Reviews·Roger BouillonMarie Demay
Sep 17, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A W Norman
Jun 3, 1999·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·H A PedrozoB D Boyan
Sep 7, 2000·Biochemical and Biophysical Research Communications·D TeegardenL Shi
Feb 24, 2009·Fertility and Sterility·Touraj MahmoudiZahra Ezabadi
Dec 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M UchidaJ W Pike
Feb 1, 1994·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T H ChenD M Shoback
Jul 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·D M ShobackR M Johnson
Sep 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B GrosseM Lieberherr
Nov 15, 2002·Molecular and Cellular Endocrinology·Anthony W NormanHelen L Henry
Jul 10, 2012·Journal of Diabetes and Its Complications·Dilnasheen MeerzaJamal Ahmed
Mar 1, 1995·Journal of Pharmaceutical Sciences·N SurendranJ Blanchard
Aug 21, 2009·Bioorganic & Medicinal Chemistry Letters·Daisuke SawadaAtsushi Kittaka
Apr 7, 2007·Bioorganic & Medicinal Chemistry·Laura Sánchez-AbellaVicente Gotor
Nov 27, 2004·Mechanisms of Ageing and Development·Ana Russo de Boland
Jul 1, 2004·The Journal of Steroid Biochemistry and Molecular Biology·Claudia GentiliAna Russo de Boland
Jul 15, 1992·Biochemical and Biophysical Research Communications·L X ZhouA W Norman
Jan 13, 2006·Biochemical and Biophysical Research Communications·P ForsellL Knutson
Jun 19, 2012·Ageing Research Reviews·Verónica Gonzalez Pardo, Ana Russo de Boland
May 14, 2011·Life Sciences·Leila ZanattaFátima Regina Mena Barreto Silva
Feb 1, 2008·Diabetes Care·Ian H de BoerUNKNOWN Women's Health Initiative Investigators
Oct 14, 2005·American Journal of Physiology. Cell Physiology·Joel J BerghMary C Farach-Carson

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