Infra-red (ATR/FT) spectroscopic study on the conformational structure of the isolated human gastric mucus pretreated with ethanol

Journal of Gastroenterology and Hepatology
S Y LinR C Liang

Abstract

In order to investigate the conformational structure of isolated human gastric mucus gel pretreated with or without ethanol, gel samples were determined by attenuated total reflection/Fourier transform-infra-red (ATR/FT-IR) microspectroscopy. The result indicates that the secondary structure-dependent amide I band and the glycoprotein region were significantly different in the gastric mucus gels pretreated with and without 40% ethanol. The structural composition of beta-sheet structure (1640-1600 cm-1) increased from 38.48 to 55.08% (+16.6%) after 6-hour pretreatment with 40% ethanol, but the beta-turn structure, (1660-1700 cm-1) decreased from 41.38 to 24.29% (-17.05%). The peak area ranging from 1180 to 1000 cm-1, assigned to the glycoprotein region, was also different after pretreatment with ethanol for 6 h. The higher peak area of the carbohydrate band was obtained in the frequency region between 1000 and 1040 cm-1 and 1100 and 1180 cm-1 for mucus gel pretreated with 40% ethanol. However, the peak area ranging from 1100 to 1040 cm-1 mainly due to the symmetric phosphate stretching mode of proteins was somewhat lower for the ethanol-pretreated mucus gel than the native mucus gel. This result strongly reveals that ethanol sig...Continue Reading

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Citations

Jan 6, 2000·Postgraduate Medical Journal·S Badvie
Oct 15, 2013·International Journal of Nanomedicine·Shengli DongGaopeng Li
May 7, 2011·World Journal of Gastroenterology : WJG·Yong-Song GuanZi La
Jan 1, 2014·World Journal of Gastroenterology : WJG·Qing HeAn-Ren Kuang
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Jul 18, 2009·Journal of Agricultural and Food Chemistry·Warren Miner-WilliamsMalcolm F Fuller

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