Infrared laser desorption and ionization of polypeptides from a polyacrylamide gel

Journal of Mass Spectrometry : JMS
M Baltz-KnorrR F Haglund

Abstract

We observed direct desorption and ionization of angiotensin II and bovine insulin from a frozen polyacrylamide gel without the addition of an exogenous matrix, using picosecond pulses from a tunable, mid-infrared free-electron laser tuned to strong absorption bands of the gel. At 5.7, 5.9, 6.1 and 6.3 microm we were able to desorb and ionize both analyte molecules, with the strongest analyte signal generated at 5.9 microm. However, no analyte signal was observed at 5.5 microm. Consistent with a previous report, we did not observe ions of either polypeptide at 2.9 microm, in spite of strong overall absorption. We discuss the implications of this wavelength-dependent ionization, including possible ablation mechanisms and energy partitioning between competing vibrational modes.

References

May 23, 1995·Proceedings of the National Academy of Sciences of the United States of America·K R ClauserA L Burlingame
Jul 9, 1996·Proceedings of the National Academy of Sciences of the United States of America·S BerkenkampF Hillenkamp
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Jan 14, 2000·Journal of Mass Spectrometry : JMS·J D Sheffer, K K Murray
Nov 20, 2001·Chemical Reviews·K B Tomer

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Citations

Feb 17, 2006·Journal of the American Society for Mass Spectrometry·Yichuan XuKermit K Murray
Nov 1, 2008·Expert Opinion on Drug Discovery·Richard F WinkleSanjiv Sharma
Mar 21, 2006·Rapid Communications in Mass Spectrometry : RCM·Shelley N JacksonKermit K Murray
Jul 12, 2002·Journal of Mass Spectrometry : JMS
Feb 14, 2004·Analytical Chemistry·Yichuan XuKermit K Murray

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