Infrared microscopic imaging of bone: spatial distribution of CO3(2-)

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
H Ou-YangRichard Mendelsohn

Abstract

This article describes a novel technology for quantitative determination of the spatial distribution of CO3(2-) substitution in bone mineral using infrared (IR) imaging at approximately 6 microm spatial resolution. This novel technology consists of an IR array detector of 64 x 64 elements mapped to a 400 microm x 400 microm spot at the focal plane of an IR microscope. During each scan, a complete IR spectrum is acquired from each element in the array. The variation of any IR parameter across the array may be mapped. In the current study, a linear relationship was observed between the band area or the peak height ratio of the CO3(2-) v3 contour at 1415 cm(-1) to the PO4(3-) v1,v3 contour in a series of synthetic carbonated apatites. The correlation coefficient between the spectroscopically and analytically determined ratios (R2 = 0.989) attests to the practical utility of this IR area ratio for determination of bone CO3(2-) levels. The relationship forms the basis for the determination of CO3(2-) in tissue sections using IR imaging. In four images of trabecular bone the average CO3(2-) levels were 5.95 wt% (2298 data points), 6.67% (2040 data points), 6.66% (1176 data points), and 6.73% (2256 data points) with an overall average...Continue Reading

References

Jul 25, 1975·Calcified Tissue Research·N C BlumenthalA S Posner
Aug 1, 1992·Calcified Tissue International·R G Handschin, W B Stern
Sep 1, 1987·Calcified Tissue International·R LegrosG Bonel
Oct 1, 1988·Calcified Tissue International·J E HarriesS S Hasnain
Jul 1, 1985·Calcified Tissue International·M KnuuttilaS Lammi
Apr 24, 1965·Nature·R Zapanta-LeGeros
Jan 1, 1967·Calcified Tissue Research·J D Termine, A S Posner
Jan 1, 1984·Caries Research·J D FeatherstoneR Z LeGeros
Dec 1, 1996·Calcified Tissue International·E P PaschalisA L Boskey
Jan 1, 1996·Connective Tissue Research·S J GadaletaR Mendelsohn
Feb 12, 1998·Calcified Tissue International·E P PaschalisA L Boskey
Mar 25, 1999·Calcified Tissue International·A A BaigM Otsuka
Sep 17, 1999·Journal of Biomedical Materials Research·Y DoiY Iwayama

❮ Previous
Next ❯

Citations

Jul 20, 2006·Journal of the Royal Society, Interface·A A van ApeldoornC A van Blitterswijk
Jun 7, 2007·Calcified Tissue International·Ayorinde AwonusiMary M J Tecklenburg
May 14, 2009·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·J ShenJ M Hu
Dec 6, 2005·Analytical and Bioanalytical Chemistry·Wolfram StellerChristoph Krafft
Jan 6, 2007·Urological Research·Jennifer C AndersonAndré J Sommer
Oct 19, 2005·Archives of Dermatological Research·Ming-Tzen LiuShan-Yang Lin
Sep 1, 2005·Graefe's Archive for Clinical and Experimental Ophthalmology = Albrecht Von Graefes Archiv Für Klinische Und Experimentelle Ophthalmologie·Ko-Hua ChenShan-Yang Lin
Dec 30, 2014·Proceedings of the National Academy of Sciences of the United States of America·Jinhui TaoJames J De Yoreo
May 13, 2006·Vibrational Spectroscopy·Adele L Boskey, Richard Mendelsohn
Feb 6, 2017·Calcified Tissue International·Eric AguadoDaniel Chappard
Apr 6, 2017·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·E P PaschalisK Klaushofer
May 17, 2006·Technology in Cancer Research & Treatment·Christoph KrafftReiner Salzer
Dec 28, 2017·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Heather B HuntEve Donnelly
May 5, 2011·Dermatology : International Journal for Clinical and Investigative Dermatology·Dau-Ming NiuChih-Cheng Lin
Jul 21, 2004·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Shan-Yang LinShun-Li Wang
Mar 29, 2005·Nature Biotechnology·Daniel C FernandezIra W Levin
Sep 10, 2010·Wiley Interdisciplinary Reviews. Nanomedicine and Nanobiotechnology·Elia Beniash
Oct 19, 2005·Journal of Biomedical Optics·Adele Boskey, Richard Mendelsohn
Oct 14, 2005·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Osamu SuzukiTakenobu Katagiri
Jun 7, 2019·International Journal of Legal Medicine·Tamara LeskovarMatija Črešnar
Dec 5, 2015·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Adele L BoskeyRobert R Recker
Mar 28, 2019·Journal of Biomedical Materials Research. Part a·Mehdi EbrahimiNaruporn Monmaturapoj
Aug 15, 2018·Scientific Reports·Gregorio Dal SassoGilberto Artioli
Jan 14, 2017·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Marina RodioRomuald Intartaglia
Apr 10, 2003·Journal of Biomedical Optics·Catherine P TarnowskiMark A Moore
Feb 26, 2020·Genetics, Selection, Evolution : GSE·Dirk-Jan De KoningIan C Dunn
Dec 20, 2018·Calcified Tissue International·Sity Aishah MansurGuillaume Mabilleau
Oct 11, 2020·Microscopy Research and Technique·Vineet KumarHarsukh P Gajera

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.