Infrared Microspectroscopy of Bionanomaterials (Diatoms) with Careful Evaluation of Void Effects

Applied Spectroscopy
Leila AlipourOsamu Oku

Abstract

In order to characterize a representative natural bionanomaterial, present day centric diatom samples (diameter, 175-310 µm) have been analyzed and imaged by infrared (IR) micro-spectroscopy and scanning electron microscopy (SEM). Because diatom silica frustules have complex microscopic morphology, including many void areas such as micro- or nano-pores, the effects of voids on the spectral band shapes were first evaluated. With increasing void area percentage, 1220 cm(-1)/1070 cm(-1) peak height ratio (Si-O polymerization index) increases and 950 cm(-1)/800 cm(-1) peak height ratio (Si-OH/Si-O-Si) decreases, both approaching 1. Based on the void area percentage of representative diatom samples determined using SEM image analyses (51.5% to 20.5%) and spectral simulation, the 1220 cm(-1)/1070 cm(-1) ratios of diatom samples are sometimes affected by the void effect, but the 950 cm(-1)/800 cm(-1) ratios can indicate real structural information of silica. This void effect should be carefully evaluated for IR micro-spectroscopy of micro-nano-porous materials. Maturity of diatom specimens may be evaluated from: (1) void area percentages determined by SEM; (2) average thicknesses determined by optical microscope; and (3) average value...Continue Reading

References

Jun 20, 1969·Science·T Nakajima, B E Volcani
Jul 11, 1998·Science·P G FalkowskiV Smetacek
May 14, 1999·Trends in Biotechnology·J Parkinson, R Gordon
May 24, 2003·Nature Materials·Zhiyong TangBirol Ozturk
Sep 4, 2003·Connective Tissue Research·Adele L Boskey
Apr 10, 2004·Science·Andrew S MountD Snider
Oct 6, 2005·Chemical Communications : Chem Comm·Dusan LosicNicolas H Voelcker
Jun 2, 2006·Journal of Nanoscience and Nanotechnology·Dusan LosicNicolas H Voelcker
Apr 3, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Dusan LosicNicolas H Voelcker
Apr 21, 1995·Science·P TréguerB Quéguiner
Nov 10, 2007·Current Opinion in Chemical Biology·Nils Kröger
Apr 3, 2008·Chembiochem : a European Journal of Chemical Biology·Manfred Sumper, Eike Brunner
Apr 19, 2008·Proceedings of the National Academy of Sciences of the United States of America·David J BeltonCarole C Perry
Oct 22, 2008·Chemical Reviews·Mark Hildebrand
Nov 6, 2008·Annual Review of Genetics·Nils Kröger, Nicole Poulsen
Feb 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·André ScheffelNils Kröger
Sep 29, 2011·Journal of Biomedical Materials Research. Part B, Applied Biomaterials·Qing LinChunhua Lu
Feb 18, 2014·Trends in Biotechnology·Orly LevitanPaul G Falkowski

❮ Previous
Next ❯

Citations

Oct 23, 2018·Skin Research and Technology : Official Journal of International Society for Bioengineering and the Skin (ISBS) [and] International Society for Digital Imaging of Skin (ISDIS) [and] International Society for Skin Imaging (ISSI)·Sachie Kudo, Satoru Nakashima

❮ Previous
Next ❯

Methods Mentioned

BETA
scanning electron microscopy
optical microscopy

Software Mentioned

ImageJ

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.