Infrared Nanospectroscopy of Phospholipid and Surfactin Monolayer Domains

ACS Omega
Bernd KästnerGerhard Ulm

Abstract

A main challenge in understanding the structure of a cell membrane and its interactions with drugs is the ability to chemically study the different molecular species on the nanoscale. We have achieved this for a model system consisting of mixed monolayers (MLs) of the biologically relevant phospholipid 1,2-distearoyl-sn-glycero-phosphatidylcholine and the antibiotic surfactin. By employing nano-infrared (IR) microscopy and spectroscopy in combination with atomic force microscopy imaging, it was possible to identify and chemically detect domain formation of the two constituents as well as to obtain IR spectra of these species with a spatial resolution on the nanoscale. A novel method to enhance the near-field imaging contrast of organic MLs by plasmon interferometry is proposed and demonstrated. In this technique, the organic layer is deposited on gold and ML graphene substrates, the latter of which supports propagating surface plasmons. Plasmon reflections arising from changes in the dielectric environment provided by the organic layer lead to an additional contrast mechanism. Using this approach, the interfacial region between surfactin and the phospholipid has been mapped and a transition region is identified.

References

Apr 26, 1991·Biochimica Et Biophysica Acta·J D SheppardR Laprade
May 1, 1995·Biophysical Journal·R Maget-Dana, M Ptak
Jun 23, 1998·Quarterly Reviews of Biophysics·L K Tamm, S A Tatulian
Jul 15, 1998·The Journal of Membrane Biology·D A Brown, E London
Sep 27, 2000·Physical Review Letters·R Hillenbrand, F Keilmann
Nov 7, 2000·Biochimica Et Biophysica Acta·J F Nagle, S Tristram-Nagle
Jul 12, 2002·Nature·R HillenbrandF Keilmann
Feb 4, 2003·Journal of Lipid Research·Linda J Pike
May 9, 2012·Beilstein Journal of Nanotechnology·Sergiu AmarieFritz Keilmann
Jun 19, 2012·Nano Letters·Florian HuthRainer Hillenbrand
Jun 23, 2012·Nature·Jianing ChenFrank H L Koppens
Oct 11, 2012·ACS Nano·Peining Li, Thomas Taubner
Oct 15, 2013·Nature Nanotechnology·Z FeiD N Basov
Nov 21, 2013·Journal of the American Chemical Society·Samuel BerwegerMarkus B Raschke
Dec 5, 2013·Nature Communications·Iban AmenabarRainer Hillenbrand
Jan 17, 2014·Journal of Chemical Biology·Christopher P Toseland
May 8, 2014·Proceedings of the National Academy of Sciences of the United States of America·Hans A BechtelMarkus B Raschke
Jul 16, 2014·Nano Letters·Peining LiThomas Taubner
Aug 16, 2014·Physical Review Letters·Justin A GerberMarkus B Raschke
Apr 22, 2015·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Mattias KruskopfHans W Schumacher
Jun 10, 2015·Cold Spring Harbor Perspectives in Biology·Petra Anne Levin, Esther R Angert
Jul 30, 2015·Nano Letters·Z FeiD N Basov
Dec 15, 2015·Nano Letters·Benjamin PollardRaul O Freitas
Aug 10, 2017·Optics Express·Peter HermannGerhard Ulm

❮ Previous
Next ❯

Methods Mentioned

BETA
atomic force microscopy
AFM
as
plasmon
plasmon interferometry

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.