PMID: 9630714Jun 19, 1998Paper

Infrared spectroscopic identification of the C-O stretching vibration associated with the tyrosyl Z. and D. radicals In photosystem II

Biochimica Et Biophysica Acta
S KimB A Barry

Abstract

Photosystem II (PSII) is a multisubunit complex, which catalyzes the photo-induced oxidation of water and reduction of plastoquinone. Difference Fourier-transform infrared (FT-IR) spectroscopy can be used to obtain information about the structural changes accompanying oxidation of the redox-active tyrosines, D and Z, in PSII. The focus of our work is the assignment of the 1478 cm-1 vibration, which is observable in difference infrared spectra associated with these tyrosyl radicals. The first set of FT-IR experiments is performed with continuous illumination. Use of cyanobacterial strains, in which isotopomers of tyrosine have been incorporated, supports the assignment of a positive 1478/1477 cm-1 mode to the C-O stretching vibration of the tyrosyl radicals. In negative controls, the intensity of this spectral feature decreases. The negative controls involve the use of inhibitors or site-directed mutants, in which the oxidation of Z or D is eliminated, respectively. The assignment of the 1478/1477 cm-1 mode is also based on control EPR and fluorescence measurements, which demonstrate that no detectable Fe+2QA- signal is generated under FT-IR experimental conditions. Additionally, the difference infrared spectrum, associated with...Continue Reading

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Citations

Sep 30, 2016·Photosynthesis Research·Alberto Mezzetti, Winfried Leibl
May 13, 2018·Proceedings of the National Academy of Sciences of the United States of America·Zhanjun GuoBridgette A Barry
Sep 9, 2004·Chemical Reviews·Sumitra MukhopadhyayWilliam H Armstrong
Apr 10, 2012·Journal of the American Chemical Society·Wolfram SanderElsa Sanchez-Garcia

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