Infrequent methylation of CDKN2A(MTS1/p16) and rare mutation of both CDKN2A and CDKN2B(MTS2/p15) in primary astrocytic tumours

British Journal of Cancer
E E SchmidtV P Collins

Abstract

In a series of 46 glioblastomas, 16 anaplastic astrocytomas and eight astrocytomas, all tumours retaining one or both alleles of CDKN2A (48 tumours) and CDKN2B (49 tumours) were subjected to sequence analysis (entire coding region and splice acceptor and donor sites). One glioblastoma with hemizygous deletion of CDKN2A showed a missense mutation in exon 2 (codon 83) that would result in the substitution of tyrosine for histidine in the protein. None of the tumours retaining alleles of CDKN2B showed mutations of this gene. Glioblastomas with retention of both alleles of CDKN2A (14 tumours) and CDKN2B (16 tumours) expressed transcripts for these genes. In contrast, 7/13 glioblastomas with hemizygous deletions of CDKN2A and 8/11 glioblastomas with hemizygous deletions of CDKN2B showed no or weak expression. Anaplastic astrocytomas and astrocytomas showed a considerable variation in the expression of both genes, regardless of whether they retained one or two copies of the genes. The methylation status of the 5' CpG island of the CDKN2A gene was studied in all 15 tumours retaining only one allele of CDKN2A as well as in the six tumours showing no significant expression of transcript despite their retaining both CDKN2A alleles. Three...Continue Reading

Citations

Nov 4, 2000·International Journal of Cancer. Journal International Du Cancer·S BortolottoD Schiffer
Apr 17, 2001·Brain Pathology·M NakamuraH Ohgaki
Jul 17, 2001·Journal of Neurosurgery·T KouhataK Tabuchi
Mar 10, 2006·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·David A ReardonDarell D Bigner
Sep 26, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·M K SibinG K Chetan
Apr 18, 1998·Brain Pathology·J M CobbersG Reifenberger
Apr 17, 2001·International Journal of Cancer. Journal International Du Cancer·R BüschgesG Reifenberger
May 2, 2003·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Tetsuya Shiraishi, Kazuo Tabuchi
Jun 12, 2010·Acta Biochimica Et Biophysica Sinica·Zhuanyi YangYihua Rao
Aug 7, 2001·Journal of Neuropathology and Experimental Neurology·S M DongH K Ng
Apr 16, 1998·Proceedings of the National Academy of Sciences of the United States of America·S ZhangB A Mock
May 12, 2004·Nature Reviews. Drug Discovery·Jeremy N Rich, Darell D Bigner
Jan 28, 2005·Journal of Neuro-oncology·Koichi IchimuraV Peter Collins
Apr 17, 2008·Brain Tumor Pathology·Yoshiyuki TanakaToru Itakura
Mar 1, 2021·BMC Medical Informatics and Decision Making·Claire Jean-QuartierAndreas Holzinger
Jun 16, 2001·Seminars in Cancer Biology·K M Reilly, T Jacks
Nov 21, 1998·Biochimica Et Biophysica Acta·M Ruas, G Peters
Jan 5, 2002·Hematology/oncology Clinics of North America·S A Rempel
Apr 12, 2003·Neuroimaging Clinics of North America·Ian F PollackFrank Lieberman
Mar 26, 2010·Journal of Experimental & Clinical Cancer Research : CR·Zhuanyi YangYanjin Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Astrocytes

Astrocytes are glial cells that support the blood-brain barrier, facilitate neurotransmission, provide nutrients to neurons, and help repair damaged nervous tissues. Here is the latest research.