Inhabiting plant roots, nematodes, and truffles-Polyphilus, a new helotialean genus with two globally distributed species

Mycologia
Samad AshrafiGábor M Kovács

Abstract

Fungal root endophytes, including the common group of dark septate endophytes (DSEs), represent different taxonomic groups and potentially diverse life strategies. In this study, we investigated two unidentified helotialean lineages found previously in a study of DSE fungi of semiarid grasslands, from several other sites, and collected recently from a pezizalean truffle ascoma and eggs of the cereal cyst nematode Heterodera filipjevi. The taxonomic positions and phylogenetic relationships of 21 isolates with different hosts and geographic origins were studied in detail. Four loci, namely, nuc rDNA ITS1-5.8S-ITS2 (internal transcribed spacer [ITS]), partial 28S nuc rDNA (28S), partial 18S nuc rDNA (18S), and partial RNA polymerase II second-largest subunit (RPB2), were amplified and sequenced for molecular phylogenetic analyses. Analyses of similar ITS sequences from public databases revealed two globally distributed lineages detected in several biomes from different geographic regions. The host interaction of isolates from nematodes was examined using in vitro bioassays, which revealed that the fungi could penetrate nematode cysts and colonize eggs of H. filipjevi, confirming observations from field-collected samples. This is t...Continue Reading

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Citations

Aug 28, 2020·IMA Fungus·Jose G Maciá-VicenteOndřej Koukol
Nov 30, 2019·Frontiers in Microbiology·Dániel G KnappGábor M Kovács
Jul 11, 2020·IMA Fungus·Peter R JohnstonJeffrey P Townsend

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