Inhalable Levofloxacin Liposomes Complemented with Lysozyme for Treatment of Pulmonary Infection in Rats: Effective Antimicrobial and Antibiofilm Strategy

AAPS PharmSciTech
Purnima V GuptaMangal S Nagarsenker

Abstract

Treatment of bacterial infections becomes increasingly complicated due to increasing bacterial resistance and difficulty in developing new antimicrobial agents. Emphasis should be laid on improvising the existing treatment modalities. We studied the improved antimicrobial and antibiofilm activity of levofloxacin (LFX) and lysozyme (LYS) in microbiological studies. LFX at sub-minimum inhibitory concentration with LYS eradicated > 85% of preformed biofilm. LFX was actively loaded into the liposomes using pH gradient method and was spray-dried with LYS solution. Percent entrapment of LFX in liposome was > 80% and prolonged cumulative release of 85% LFX at the end of 12 h. In vitro lung deposition study and solid-state characterization for spray dried LFX liposome in combination with LYS (LFX liposome-LYS) was performed. Co-spray dried product had mass median aerodynamic diameter ranging < 5 μm. In pharmacodynamic study, Staphylococcus aureus infected rats were treated with LFX liposome-LYS. Lungs, bronchoalveolar lavage fluid (BALF), and nasal fluid were evaluated for microbial burden. Expression of cytokine levels in BALF and serum were also studied by ELISA. In addition, mRNA expression for lung inflammatory mediators and lung m...Continue Reading

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Citations

Aug 15, 2020·Microbiology and Molecular Biology Reviews : MMBR·Katrin Schilcher, Alexander R Horswill
Jan 7, 2021·Chemical Society Reviews·Lewis D BlackmanKatherine E S Locock
Jun 6, 2021·International Journal of Pharmaceutics·Li DingZhengrong Cui

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Methods Mentioned

BETA
Assay
dynamic light scattering
scanning electron microscopy
X-ray
enzyme-linked immunosorbent assay
PCR

Software Mentioned

CITDAS

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