Inhalation of Burkholderia thailandensis results in lethal necrotizing pneumonia in mice: a surrogate model for pneumonic melioidosis.

Transactions of the Royal Society of Tropical Medicine and Hygiene
T Eoin WestShawn J Skerrett

Abstract

Burkholderia thailandensis is closely related to B. pseudomallei, the causative agent of melioidosis, a lethal tropical disease. Burkholderia thailandensis is largely avirulent in humans and, unlike B. pseudomallei, does not require strict biocontainment conditions for study. Because it may be a useful research surrogate for B. pseudomallei, we developed a murine model of airborne B. thailandensis infection. In both C57BL/6 and BALB/c mice, deposition of 103 colony-forming units (CFU)/lung or less of B. thailandensis was non-lethal and infection was readily controlled. Compared with C57BL/6 mice, BALB/c mice exhibited modest resistance to infection after deposition of 10(4) CFU/lung. Deposition of 10(5) CFU/lung resulted in disseminated infection and was universally fatal by 3d. This dose induced robust pulmonary neutrophilia, production of inflammatory cytokines, and elevated serum markers of distant organ injury. Histology demonstrated multiple small foci of necrotizing pneumonia but lung architecture was otherwise preserved, suggesting that respiratory failure is not the cause of death. These findings demonstrate that airborne B. thailandensis infection in mice provides an accessible surrogate model of melioidosis.

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Citations

Oct 3, 2009·Infection and Immunity·T Eoin WestShawn J Skerrett
Mar 16, 2011·Infection and Immunity·Benjamin M HasselbringMark A Schell
Aug 4, 2009·Journal of Bacteriology·Josephine R ChandlerE Peter Greenberg
Jul 7, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christopher T FrenchJeff F Miller
Mar 25, 2016·Expert Review of Vaccines·Sophie A AschenbroichRobert J Hogan
Jul 22, 2015·PLoS Neglected Tropical Diseases·Ryan J BlowerMonique L van Hoek
Jan 28, 2014·Journal of Bacteriology·Charlotte MajerczykE Peter Greenberg
May 14, 2010·Natural Product Reports·Jörn Piel
May 1, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jinyong WangFabio Re
Sep 15, 2017·PLoS Neglected Tropical Diseases·Erin P PriceDavid M Wagner
Nov 10, 2012·International Journal of Experimental Pathology·T Eoin WestShawn J Skerrett
May 16, 2013·PLoS Neglected Tropical Diseases·Saja AsakrahLisa A Morici
Jan 29, 2019·Frontiers in Microbiology·Jan LenningsSandra Schwarz
Sep 26, 2020·The American Journal of Tropical Medicine and Hygiene·Anna S AmissNicole Lawrence

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