Inherent structural disorder and dimerisation of murine norovirus NS1-2 protein.

PloS One
Estelle S BakerVernon K Ward

Abstract

Human noroviruses are highly infectious viruses that cause the majority of acute, non-bacterial epidemic gastroenteritis cases worldwide. The first open reading frame of the norovirus RNA genome encodes for a polyprotein that is cleaved by the viral protease into six non-structural proteins. The first non-structural protein, NS1-2, lacks any significant sequence similarity to other viral or cellular proteins and limited information is available about the function and biophysical characteristics of this protein. Bioinformatic analyses identified an inherently disordered region (residues 1-142) in the highly divergent N-terminal region of the norovirus NS1-2 protein. Expression and purification of the NS1-2 protein of Murine norovirus confirmed these predictions by identifying several features typical of an inherently disordered protein. These were a biased amino acid composition with enrichment in the disorder promoting residues serine and proline, a lack of predicted secondary structure, a hydrophilic nature, an aberrant electrophoretic migration, an increased Stokes radius similar to that predicted for a protein from the pre-molten globule family, a high sensitivity to thermolysin proteolysis and a circular dichroism spectrum ...Continue Reading

References

Jan 6, 1981·Biochemistry·S W Provencher, J Glöckner
Dec 10, 1996·Proceedings of the National Academy of Sciences of the United States of America·A ShevchenkoM Mann
Apr 30, 1998·Genetics·J W DrakeJ F Crow
Oct 21, 1998·Journal of Theoretical Biology·K J Dawson
Feb 27, 1999·Protein Science : a Publication of the Protein Society·N SreeramaR W Woody
Nov 5, 1999·Journal of Molecular Biology·P E Wright, H J Dyson
Nov 28, 2000·Proteins·P RomeroA K Dunker
Jan 9, 2001·Clinical Microbiology Reviews·R L Atmar, M K Estes
May 31, 2001·Journal of Molecular Graphics & Modelling·A K DunkerZ Obradovic
Sep 5, 2001·Nature Biotechnology·A K Dunker, Z Obradovic
Jan 11, 2002·European Journal of Biochemistry·Vladimir N Uversky
Mar 23, 2002·Protein Science : a Publication of the Protein Society·Vladimir N Uversky
Aug 8, 2002·Journal of Molecular Evolution·Celeste J BrownA Keith Dunker
Oct 9, 2002·Trends in Biochemical Sciences·Peter Tompa
Mar 8, 2003·Science·Stephanie M KarstHerbert W Virgin
Jun 26, 2003·Nucleic Acids Research·Rune LindingToby J Gibson
Nov 8, 2003·Structure·Rune LindingRobert B Russell
Mar 2, 2005·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Carmelann ZintzDavid O Matson
Mar 2, 2005·Nature Reviews. Molecular Cell Biology·H Jane Dyson, Peter E Wright
Jun 28, 2005·Nucleic Acids Research·Kevin BrysonDavid T Jones
Aug 12, 2005·Journal of Molecular Recognition : JMR·Vladimir N UverskyA Keith Dunker
Oct 13, 2005·The FEBS Journal·A Keith DunkerVladimir N Uversky
Nov 16, 2005·Proteins·Véronique Receveur-BréchotSonia Longhi
Dec 14, 2005·Virology·Du-Ping ZhengStephan S Monroe
May 16, 2006·Journal of Virology·Christiane E WobusHerbert W Virgin
May 24, 2006·Proceedings of the National Academy of Sciences of the United States of America·Pedro R RomeroA Keith Dunker
Dec 6, 2006·Nucleic Acids Research·Megan SickmeierA Keith Dunker
Dec 13, 2006·Biophysical Journal·Predrag RadivojacA Keith Dunker

❮ Previous
Next ❯

Citations

Nov 8, 2014·Science·Melissa K JonesStephanie M Karst
Jul 23, 2013·PloS One·Christopher WilsonEmmanuel J Chang
Aug 24, 2012·Journal of Virology·Lucy ThorneIan Goodfellow
Apr 4, 2014·Journal of Virology·Mariam B Gonzalez-HernandezChristiane E Wobus
Oct 28, 2017·PLoS Pathogens·Sylvie Y DoerflingerVolker Lohmann
Jun 12, 2019·Nature Microbiology·Jessica A NeilKen Cadwell
Jul 19, 2020·Protein Expression and Purification·Johann HabersetzerSonia Fieulaine
Aug 3, 2021·Frontiers in Microbiology·Elena SmertinaMichael Frese
Aug 29, 2021·Viruses·Louisa F Ludwig-BegallEtienne Thiry

❮ Previous
Next ❯

Datasets Mentioned

BETA
EU360814

Methods Mentioned

BETA
x-ray
gel
Profiler
two-hybrid
bacterial
dynamic light scattering
circular dichroism
glycosylation
PCR
size exclusion chromatography

Software Mentioned

PSIPRED Protein Structure Prediction
PONDR®
Dynamics
PSIPRED Protein Structure Prediction Server
MegAlign
Composition Profiler
Me taserver of D der ( MeDor
DNASTAR
ClustalW
PSIPRED

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.