PMID: 18408353Apr 15, 2008Paper

Inheritance of histone H3 methylation in reprogramming of somatic nuclei following nuclear transfer

The Journal of Reproduction and Development
Gen-Bao ShaoDe-Sheng Xiao

Abstract

Successful cloning requires reprogramming of epigenetic information of the somatic nucleus to an embryonic state. However, the molecular mechanisms regarding epigenetic reprogramming of the somatic chromatin are unclear. Herein, we transferred NIH3T3 cell nuclei into enucleated mouse oocytes and evaluated the histone H3 dimethyl-lysine 4 (H3K4me2) dynamics by immunocytochemistry. A low level of H3K4me2 in the somatic chromatin was maintained in pseudo-pronuclei. Unlike in vitro fertilized (IVF) embryos, the methylation level of nuclear transfer (NT) embryos was significantly increased at the 8-cell stage. NT embryos showed lower H3K4me2 intensity than IVF embryos at the 2-cell stage, which is when the mouse embryonic genome is activated. Moreover, the H3K4me2 signal was weak in the recloned embryos derived from single blastomeres of the NT embryos, whereas it was intense in those from IVF embryos. Two imprinted genes, U2afbp-rs and Xist, were abnormally transcribed in cloned embryos compared with IVF embryos, and this was partly correlated to the H3K4me2 level. Our results suggest that abnormal reprogramming of epigenetic markers such as histone acetylation and methylation may lead to dysregualtion of gene expression in cloned ...Continue Reading

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Citations

Mar 13, 2014·In Vitro Cellular & Developmental Biology. Animal·Gen-Bao ShaoJian-Rong Sang
Oct 10, 2009·The Journal of Reproduction and Development·Ken-Ichi YamanakaMasashi Takahashi
Apr 29, 2010·Yi chuan = Hereditas·Gen-Bao ShaoJian-Rong Sang
Aug 10, 2012·Reproductive Biomedicine Online·Lei SunHou-Qi Liu
Aug 28, 2012·Animal Reproduction Science·Karlla MasonNathalie Beaujean
Aug 30, 2016·The Journal of Reproduction and Development·Fang ZengZicong Li
Jan 5, 2014·Reproduction, Fertility, and Development·Gen-Bao ShaoWan-Xin Peng

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