Inheritance of Histones H3 and H4 during DNA Replication In Vitro

Cell Reports
Egbert Vincent MadambaNicole Jane Francis

Abstract

Nucleosomes are believed to carry epigenetic information through the cell cycle, including through DNA replication. It has been known for decades that parental histones are reassembled on newly replicated chromatin, but the mechanisms underlying histone inheritance and dispersal during DNA replication are not fully understood. We monitored the fate of histones H3 or H4 from a single nucleosome through DNA replication in two in vitro systems. In the SV40 system, histones assembled on a single nucleosome positioning sequence can be inherited by their own daughter DNA but are dispersed from their original location. In Xenopus laevis extracts, histones are dynamic, and nucleosomes are repositioned independent of and prior to DNA replication. Nevertheless, a high fraction of histones H3 and H4 that are inherited through DNA replication remains near its starting location. Thus, inheritance of histone proteins and their dispersal can be mechanistically uncoupled.

Citations

Sep 6, 2018·Annual Review of Genetics·Prerna GroverEric I Campos
May 28, 2019·Genes & Development·Jia-Ray YuDanny Reinberg
Sep 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Steven Henikoff, Kami Ahmad
Sep 13, 2019·Proceedings of the National Academy of Sciences of the United States of America·Gavin Schlissel, Jasper Rine
Apr 25, 2019·Essays in Biochemistry·Alonso J PardalAndrew J Bowman
Apr 2, 2020·Nature Cell Biology·Kathleen R Stewart-MorganAnja Groth
Aug 28, 2020·Frontiers in Cell and Developmental Biology·William A Scott, Eric I Campos
Jan 28, 2021·Nature Reviews. Genetics·Thelma M EscobarDanny Reinberg
Jul 7, 2020·Journal of Molecular Biology·Jin Joo KangNicole J Francis

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