PMID: 2504871Jul 1, 1989Paper

Inhibin and activin-like activity in fluids from male and female gonads: different molecular weight forms and bioactivity/immunoactivity ratios

The Journal of Endocrinology
A J GrootenhuisF H de Jong

Abstract

The existence of various molecular weight forms of inhibin in ovarian follicular fluid has been reported earlier, while there is no information on the form of inhibin in testicular tissue. Inhibin bioactivity was therefore estimated in eluates of slices, obtained after SDS-PAGE of rat testicular and ovarian homogenates, rat Sertoli cell-conditioned medium (rSCCM) and bovine ovarian follicular fluid (bFF). The only form of inhibin detected in testes from 22-day-old rats and in rSCCM was a 30 kDa protein. In rat ovarian extracts, larger forms of inhibin were also found as well as the predominant 30 kDa form. An activin-like activity was found in the 25 kDa SDS-PAGE eluates of both rSCCM and ovarian homogenates, which caused a dose-dependent increase of FSH release from cultured pituitary cells. Activin-like activity and several forms of inhibin were found in bFF after SDS-PAGE. After purification of inhibin from bFF using dye affinity, anion-exchange, lentil lectin affinity chromatography and a subsequent reversed phase chromatography step, two pools of inhibin activity were obtained. These were separated by SDS-PAGE revealing 30 and 58 kDa inhibin forms. The immunoactivity of these forms of inhibin was then estimated using antib...Continue Reading

Citations

Aug 1, 1995·The Prostate·K KharbandaS Duraiswami
Sep 27, 1993·Journal of Immunological Methods·W L WongJ A Lofgren
Mar 26, 1990·Molecular and Cellular Endocrinology·A J GrootenhuisF H de Jong
Aug 20, 1990·Molecular and Cellular Endocrinology·H F MarzoukS W Lamberts
Jan 1, 1992·Molecular and Cellular Endocrinology·J P de WinterF H de Jong
Dec 20, 1990·The Journal of Steroid Biochemistry and Molecular Biology·F H De JongJ G Klijn
Nov 7, 2000·Molecular and Cellular Endocrinology·A MeinhardtD M de Kretser
Jul 14, 2001·Molecular and Cellular Endocrinology·D M de KretserN G Wreford
Apr 25, 2000·Molecular and Cellular Endocrinology·D M de KretserK A Loveland
Dec 1, 1991·Molecular and Cellular Neurosciences·P S Lapolt, A J Hsueh
Oct 29, 2017·Reproduction in Domestic Animals = Zuchthygiene·Y WuJ Huang
Jun 1, 1995·Livestock Production Science·R H Meloen

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