PMID: 2111253Mar 26, 1990Paper

Inhibin in immature rat Sertoli cell conditioned medium: a 32 kDa alpha beta-B dimer

Molecular and Cellular Endocrinology
A J GrootenhuisF H de Jong

Abstract

Conditioned medium of cultured Sertoli cells from 21-day-old rats was used as starting material for the isolation of inhibin. Inhibin activity was monitored by the dose dependent suppression of the follicle-stimulating hormone release of cultured rat pituitary cells. Sodium dodecyl sulphate-polyacrylamide gel electrophoresis analysis of the highly purified inhibin preparation revealed a 32 kDa protein after silver staining, which could be separated in subunits of 18 kDa and 12 kDa after reduction. Western blot analysis with an antibody recognizing the 22 N-terminal amino acids of the alpha-subunit of 32 kDa bovine inhibin confirmed the presence of a 32 kDa inhibin molecule under non-reducing conditions, whereas an 18 kDa alpha-subunit was found after reduction. An antibody recognizing the beta-A subunit of inhibin did not yield a signal after Western blotting. N-terminal amino acid sequence analysis of two highly purified preparations of inhibin obtained using different methods yielded the sequence predicted for a 32 kDa alpha beta-B dimer on basis of cDNA nucleotide sequence. This result is in agreement with the large excess of beta-B over beta-A mRNA in the rat testis.

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Citations

Aug 1, 1995·The Prostate·K KharbandaS Duraiswami
Apr 1, 1992·Baillière's Clinical Endocrinology and Metabolism·D M RobertsonD M de Kretser
Oct 1, 1996·British Journal of Obstetrics and Gynaecology·E M Wallace, D L Healy
Dec 16, 1998·The Journal of Clinical Endocrinology and Metabolism·A M AnderssonN E Skakkebaek
Sep 9, 2019·Cell and Tissue Research·Sakineh PanahiReza Mastery Farahani
Jul 23, 2014·Endocrine Reviews·Yogeshwar MakanjiTeresa K Woodruff
Aug 1, 1997·The Journal of Clinical Endocrinology and Metabolism·T M PlantA S McNeilly
Jun 23, 2016·Cold Spring Harbor Perspectives in Biology·Maria Namwanje, Chester W Brown

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