Inhibins, activins, and follistatins: expression of mRNAs and cellular localization in tissues from men with benign prostatic hyperplasia

The Prostate
T Z ThomasG P Risbridger

Abstract

The transforming growth factor beta (TGF beta) superfamily of growth factors includes activins and inhibins, which have been shown to be present in the rat ventral prostate, and human prostate tumor cell lines, although their localization in benign prostatic hyperplasia (BPH) tissue is currently unknown. BPH tissues were obtained at surgery, and the mRNA expression for the inhibin alpha, beta A, beta B subunits, the putative activin beta C subunit, the activin type II receptor (ActRII), and the activin binding protein, follistatin, was determined by reverse transcription polymerase chain reaction (RT-PCR) and Southern blot analysis. Antibodies specific for alpha, beta A, beta B, activin A, and follistatin were used to determine the localization of these proteins in BPH tissue specimens. Southern blot analysis confirmed that mRNA for ActRII, beta C subunit, and follistatin was present in all biopsy samples assayed. However, alpha, beta A, and beta B subunit mRNA expression was variable between patient samples. Immunohistochemistry demonstrated the predominant localization of beta A, beta B, and activin A proteins to the epithelium of BPH tissues. No immunoreactivity for the inhibin alpha subunit was detected; follistatin immunor...Continue Reading

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Citations

Jul 27, 2011·Molecular and Cellular Endocrinology·Elspeth Gold, Gail Risbridger
Oct 21, 2004·Cancer·David G BostwickBarry Timms
Dec 11, 2003·The Journal of Urology·G P RisbridgerD M Peehl
Jul 14, 2004·Molecular and Cellular Endocrinology·Elspeth J GoldJean S Fleming
Sep 29, 2004·Molecular and Cellular Endocrinology·G P RisbridgerD M Peehl
Mar 25, 2015·Prostate Cancer and Prostatic Diseases·A WekesaR W Watson
Aug 24, 2001·Developmental Biology·B CancillaG P Risbridger
Sep 15, 2001·Applied Immunohistochemistry & Molecular Morphology : AIMM·E Y Cho, G H Ahn
Dec 13, 2018·Physiological Reviews·Enrrico BloiseFernando M Reis
Aug 27, 2005·The Journal of Biological Chemistry·Guangchun ChenHajime Nawata
Dec 20, 2003·Molecular Endocrinology·Jennifer L CareyShyamala Maheswaran
Jul 14, 2010·Clinical & Experimental Metastasis·Francesca Maria TumminelloGaetano Leto
Dec 12, 2001·Endocrine Reviews·G P RisbridgerD M Robertson
Mar 5, 2013·Targeted Oncology·Maria Vittoria SepportaGaetano Leto

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