Inhibition and restimulation by insulin of cellular autophagy in distal tubular cells of the kidney in early diabetic rats

Kidney & Blood Pressure Research
K HanU Pfeifer

Abstract

Cellular autophagy in the cells of distal tubular segments (DT cells) of the kidney cortex in streptozotocin-diabetic rats was evaluated by quantitative electron microscopy. Five days after streptozotocin administration, volume and numerical densities of autophagic vacuoles (AVs) in DT cells were reduced by 56 and 57%, respectively. The correction of the diabetic state by insulin injection reversed the inhibition of cellular autophagy. Volume and numerical densities of AVs increased by 97 and 53%, respectively. Endogenous insulin replacement by islet transplantation showed the same effect on cellular autophagy. Volume and numerical densities of AVs increased by 82 and 34%, respectively, as compared with sham-operated diabetic animals. The data show that, during diabetic kidney hypertrophy, the cellular autophagy is inhibited in DT cells to the same extent as in proximal tubular cells, suggesting that DT cells contribute in a balanced manner to hypertrophic growth of the kidney cortex. Similarly, DT cells are involved in the catabolic reaction, i.e., stimulation of autophagy, after metabolic correction of the diabetic state by insulin.

Citations

May 7, 2013·Antioxidants & Redox Signaling·Zhibo Wang, Mary E Choi
Feb 4, 2014·Seminars in Nephrology·Yan Ding, Mary E Choi
Aug 15, 2012·Immunological Reviews·Cees J TackMihai G Netea
Aug 25, 2015·Journal of Diabetes and Its Complications·Ying XuQiang Wan
Apr 14, 2012·Autophagy·Satoru KobayashiQiangrong Liang
Jun 14, 2012·Autophagy·Tobias B HuberZheng Dong
Feb 11, 2015·DNA and Cell Biology·Adam D Barlow, David C Thomas
Jan 14, 2017·Renal Failure·Xu DengAihua Zhang
Oct 29, 2014·The Journal of Endocrinology·Yan Ding, Mary E Choi
Sep 6, 2017·Cellular and Molecular Life Sciences : CMLS·Danyi YangZheng Dong
Apr 9, 2020·Diabetes, Obesity & Metabolism·Jonatan Barrera-Chimal, Frédéric Jaisser
May 26, 2020·BioFactors·Sasivimon Promsan, Anusorn Lungkaphin
May 27, 2020·International Journal of Nephrology·Suzan A KhodirOmnia Ameen

Related Concepts

Metazoa
Programmed Cell Death, Type II
Streptozotocin Diabetes
Hypoglycemic Effect
Insulin B Chain
Distal Convoluted Renal Tubule Structure
Electron Microscopy
Rats, Inbred Lew
Rats, Laboratory

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