Inhibition by 5'-methylthioadenosine of cell growth and tyrosine kinase activity stimulated by fibroblast growth factor receptor in human gliomas

Journal of Neurosurgery
K MiyajiK Kaba

Abstract

Stimulation of three human glioma cell lines with basic fibroblast growth factor (bFGF) led to the enhancement of cell growth and the rapid tyrosine phosphorylation of cellular proteins, including major substrates of 90 kD. A methyltransferase inhibitor, 5'-methylthioadenosine (MTA), inhibited dose dependently the bFGF-stimulated cell growth and protein tyrosine phosphorylation in glioma cells by blocking both receptor autophosphorylation and substrate phosphorylation, as shown by immunoblotting with antiphosphotyrosine antibodies and cross-linking bFGF to receptors. The antiproliferative activity of MTA correlated quantitatively with its potency as an inhibitor of bFGF-stimulated protein tyrosine kinase activity. The methyltransferase inhibitor MTA had no effect on either epidermal growth factor- or platelet-derived growth factor-stimulated protein tyrosine phosphorylation in glioma cells, but inhibited specifically bFGF-stimulated protein tyrosine kinase activity. The concentration of MTA required for inhibition of protein methylation correlated well with the concentration required for inhibition of bFGF-stimulated cell growth and protein tyrosine phosphorylation. Because MTA had no effect on numbers and dissociation constant...Continue Reading

References

Jan 1, 1992·Progress in Growth Factor Research·J PartanenK Alitalo
Mar 1, 1991·Journal of Neurosurgery·T MatsumotoK Miyaji
Apr 20, 1990·Cell·A Ullrich, J Schlessinger
Aug 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·E B Pasquale
Sep 1, 1990·Molecular and Cellular Biology·D E JohnsonL T Williams
May 1, 1990·Neurosurgery·T LichtorM E Gurney
May 1, 1989·Molecular and Cellular Biology·R FrieselT Maciag
Jul 31, 1987·Biochemical and Biophysical Research Communications·M IwaneK Igarashi
Apr 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·I VlodavskyM Klagsbrun
Jan 1, 1985·Annual Review of Biochemistry·S Clarke
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T HoffmanJ Axelrod
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·F YamaguchiR S Morrison

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Citations

May 5, 2012·American Journal of Physiology. Gastrointestinal and Liver Physiology·Nancy M BenightDouglas G Burrin
Mar 8, 2005·Journal of Theoretical Biology·Kimberly Forsten-WilliamsMatthew A Nugent
Feb 18, 2003·Molecular and Cellular Biology·Joost H A MartensAlt Zantema
Feb 14, 2006·American Journal of Physiology. Gastrointestinal and Liver Physiology·Eduardo AnsorenaMaría J Iraburu

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Methods Mentioned

BETA
antisense oligodeoxynucleotides
electrophoresis
immunoprecipitation
nuclear translocation

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