Inhibition by aphidicolin of cell cycle progression and DNA replication in sea urchin embryos

Journal of Cellular Physiology
S IkegamiY Mano

Abstract

We have recently found that aphidicolin, a tetracyclic diterpene-tetraol produced by several fungi, blocks DNA synthesis of sea urchin embryos by interfering with the activity of DNA polyermase alpha. These cells fail to proliferate in the presence of aphidicolin. In continuation of these studies, we determined the drug-sensitive stage in the first cell cycle of the sea urchin Clypeaster japonicus embryo. In continuous exposure to aphidicolin (2 micrograms/ml) from five minutes after fertilization, mitotic division of the embryo was completely suppressed. Embryos were exposed to the drug at progressively later intervals and their capability for cytokinesis was examined. Evidence was thereby obtained that aphidicolin acts at the S-period to inhibit DNA synthesis resulting in developmental arrest of the embryo.

References

Jun 29, 1978·Biochemical and Biophysical Research Communications·M OhashiS Ikegami
Apr 1, 1975·Experimental Cell Research·C K Mathews
Jan 1, 1977·Annual Review of Biochemistry·N R Cozzarelli
Mar 30, 1979·Biochemical and Biophysical Research Communications·F HanaokaM Yamada
Jul 1, 1979·European Journal of Biochemistry·M OguroS Ikegami
Jun 16, 1975·Biochemical and Biophysical Research Communications·B B Gourlie, A A Infante
Sep 1, 1973·Antimicrobial Agents and Chemotherapy·R A BucknallB Hesp
Mar 1, 1974·Canadian Journal of Microbiology·A N Starratt, S R Loschiavo

❮ Previous
Next ❯

Citations

Jul 30, 1981·Developmental Biology·H NaganoS Ikegami
Nov 1, 1986·The Journal of Cell Biology·G SluderC L Rieder
Dec 9, 2004·Biology of the Cell·Jolanta KisielewskaMichael Whitaker
Jan 1, 1984·Differentiation; Research in Biological Diversity·W L Dean, J Rossant
Jan 1, 1981·Differentiation; Research in Biological Diversity·J BrachetH Alexandre
Jun 1, 1995·Molecular Reproduction and Development·M GagnéM A Sirard
Jan 1, 1984·Journal of Cellular Physiology·D NishiokaM K Sgagias
Oct 1, 1987·The Journal of Experimental Zoology·G Sluder, K Lewis
Aug 1, 1985·The Journal of Experimental Zoology·A SpindleR A Pedersen
Oct 1, 1982·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·G IliakisP Bryant
Jan 7, 2004·Development, Growth & Differentiation·Hiromi Takata, Tetsuya Kominami
Oct 1, 1986·Roux's Archives of Developmental Biology : the Official Organ of the EDBO·Sarah K Howlett
Dec 1, 1988·Molecular and Cellular Biology·P BaumB Byers
Sep 1, 1981·Experimental Cell Research·J Brachet, B de Petrocellis

❮ Previous
Next ❯

Related Concepts

Related Feeds