PMID: 8587646Aug 1, 1995Paper

Inhibition by Cl- channel blockers of the volume-activated, diffusional mechanism of inositol transport in primary astrocytes in culture

Neurochemical Research
E GonzálezH Pasantes-Morales

Abstract

[3H]Inositol accumulated by rat brain cultured astrocytes is released when cells swell by exposure to solutions of decreased osmolarity. Activation of inositol efflux was proportional to reductions in osmolarity from 30%-70%. This volume-activated inositol efflux pathway was increased (27%) in Na(+)-free medium and decreased (22%) in Cl(-)-free medium. It was independent of extracellular Ca2+ and was reduced (30%) in the presence of the intracellular chelator [1,2-bis(o-aminophenoxy) ethane-N,N,N',N'-tetraacetic acid tetra-(acetoxymethyl)-ester] (BAPTA-AM). The inositol efflux pathway was markedly inhibited by Cl- channel blockers, which at maximal inhibitory concentrations decreased inositol efflux by 70%-83%. The potency range of the drugs was: 5-nitro-2-(3-phenylpropylamino)benzoic acid (NPPB) > 1-9, dideoxyforskolin > 4,4'-diisothiocyanatostilbene-2, 2'-disulfonic acid (DIDS) > niflumic acid. Inositol efflux was strongly inhibited by the SH blocker N-ethyl maleimide (NEM), which at 100 microM abolished inositol release. Inositol efflux can be reversed by increasing its extracellular concentration, suggesting that the efflux is mediated by a diffusional pathway whose direction is given by the concentration gradient. The inhi...Continue Reading

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Citations

Sep 1, 1999·Journal of Neuroscience Research·R E IsaacksM D Norenberg
Sep 1, 1996·Metabolic Brain Disease·H Pasantes-Morales
Oct 1, 1996·Molecular Neurobiology·S Basavappa, J C Ellory
May 23, 1998·European Journal of Pharmacology·D SongJ W Phillis
Oct 3, 2002·Journal of Neurochemistry·Stephen K FisherBernard W Agranoff
Jun 1, 1997·The American Journal of Physiology·H Pasantes-MoralesJ Morán
Mar 31, 1999·Journal of Neurochemistry·J E NovakS K Fisher
Jan 29, 1999·Physiological Reviews·B D SchultzR J Bridges
Sep 1, 1996·The American Journal of Physiology·L J GaliettaO Zegarra-Moran

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